TY - JOUR
T1 - rpm-1, a conserved neuronal gene that regulates targeting and synaptogenesis in C. elegans
AU - Schaefer, Anneliese M.
AU - Hadwiger, Gayla D.
AU - Nonet, Michael L.
N1 - Funding Information:
We thank the Jin laboratory and Goodman laboratory for sharing unpublished observations; the Jin laboratory, Pat Mink, John Spieth, and the Genome Sequencing Center for sharing data regarding the duplication of rpm-1 sequences; members of the Nonet laboratory for comments on the manuscript; Poupak Rahmani and Don Moerman for sharing deficiency breakpoint data; Yuji Kohara for EST cDNAs; Guoqiang Gu, Miriam Goodman, and Marty Chalfie for the uIs25 strain and anti-MEC-7 antibodies; and Andy Fire for GFP vectors. Some strains used in this work were obtained from the Caenorhabditis Genetics Center. A. M. S. was supported by National Institutes of Health predoctoral training grant GM07067. This work was funded by grants to M. L. N. from the Searle Scholar Program and the McKnight Foundation for Neuroscience.
PY - 2000
Y1 - 2000
N2 - Little is known of mechanisms regulating presynaptic differentiation. We identified rpm-1 in a screen for mutants with defects in patterning of a presynaptic marker at certain interneuronal synapses. The predicted RPM-1 protein contains zinc binding, RCC1, and other conserved motifs. In rpm-1 mutants, mechanosensory neurons fail to accumulate tagged vesicles, retract synaptic branches, and ectopically extend axons. Some motor neurons branch and overgrow; others show altered synaptic organization. Expression of RPM-1 in the presynaptic mechanosensory neurons is sufficient to rescue phenotypes in these cells. Certain rpm-1 phenotypes are temperature sensitive, revealing that RPM-1 function can be bypassed by maintaining mutants at the permissive temperature at stages commensurate with synapse formation in wild-type animals. These results indicate that RPM-1 functions cell autonomously during synaptogenesis to regulate neuronal morphology.
AB - Little is known of mechanisms regulating presynaptic differentiation. We identified rpm-1 in a screen for mutants with defects in patterning of a presynaptic marker at certain interneuronal synapses. The predicted RPM-1 protein contains zinc binding, RCC1, and other conserved motifs. In rpm-1 mutants, mechanosensory neurons fail to accumulate tagged vesicles, retract synaptic branches, and ectopically extend axons. Some motor neurons branch and overgrow; others show altered synaptic organization. Expression of RPM-1 in the presynaptic mechanosensory neurons is sufficient to rescue phenotypes in these cells. Certain rpm-1 phenotypes are temperature sensitive, revealing that RPM-1 function can be bypassed by maintaining mutants at the permissive temperature at stages commensurate with synapse formation in wild-type animals. These results indicate that RPM-1 functions cell autonomously during synaptogenesis to regulate neuronal morphology.
UR - http://www.scopus.com/inward/record.url?scp=0033696920&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(00)81168-X
DO - 10.1016/S0896-6273(00)81168-X
M3 - Article
C2 - 10839354
AN - SCOPUS:0033696920
SN - 0896-6273
VL - 26
SP - 345
EP - 356
JO - Neuron
JF - Neuron
IS - 2
ER -