Abstract
The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60 can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.
| Original language | English |
|---|---|
| Pages (from-to) | 3967-3972 |
| Number of pages | 6 |
| Journal | Nano Letters |
| Volume | 13 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 11 2013 |
Keywords
- Localization microscopy
- dipole emission pattern
- localization error
- molecular orientation
- rotational diffusion