Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy

Matthew D. Lew, Mikael P. Backlund, W. E. Moerner

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60 can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.

Original languageEnglish
Pages (from-to)3967-3972
Number of pages6
JournalNano Letters
Volume13
Issue number9
DOIs
StatePublished - Sep 11 2013

Keywords

  • Localization microscopy
  • dipole emission pattern
  • localization error
  • molecular orientation
  • rotational diffusion

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