@article{7f0f590bdb2741e4a66c8760627b5762,
title = "Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells",
abstract = "The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state.",
keywords = "H3K27 methylation, embryonic stem cells, polycomb proteins",
author = "Juan, {Aster H.} and Stan Wang and Ko, {Kyung Dae} and Hossein Zare and Tsai, {Pei Fang} and Xuesong Feng and Vivanco, {Karinna O.} and Ascoli, {Anthony M.} and Gustavo Gutierrez-Cruz and Jordan Krebs and Simone Sidoli and Knight, {Adam L.} and Pedersen, {Roger A.} and Garcia, {Benjamin A.} and Rafael Casellas and Jizhong Zou and Vittorio Sartorelli",
note = "Funding Information: We thank Prof. J.B. Gurdon and members of his laboratory in Cambridge, UK for hosting S.W. during the initial part of this work. We also thank the personnel of the NIAMS Sequencing, Light Imaging, and Flow Cytometry Facilities and of the NHLBI Transgenic and Pathology Cores at the National Institutes of Health (NIH). S.W. was supported in part by fellowships from the Gates Cambridge Trust and NIH-Cambridge MD/PhD Program. S.S. and B.A.G. were supported by a grant from the NIH (GM110174). This research was supported in part by the Intramural Research Program of the NIAMS at the NIH. Publisher Copyright: {\textcopyright} 2016",
year = "2016",
month = oct,
day = "25",
doi = "10.1016/j.celrep.2016.09.087",
language = "English",
volume = "17",
pages = "1369--1382",
journal = "Cell Reports",
issn = "2211-1247",
number = "5",
}