Role of the glucose cycle in control of net glucose flux in exercising humans

J. M. Weber, S. Klein, R. R. Wolfe

Research output: Contribution to journalArticle

28 Scopus citations


The hepatic glucose cycle involves the production of plasma glucose from glucose 6-phosphate and the simultaneous conversion of glucose back to glucose 6-phosphate. We have evaluated the role of the glucose cycle in the regulation of plasma glucose concentration during exercise at 70% of maximal O2 uptake and during recovery in five normal volunteers. Total glucose flux was measured by use of [2-2H]glucose (Ra2), net glucose flux through the glucose cycle was determined with [6,6-2H2]glucose (Ra6), and the rate of glucose cycling was determined by Ra2 - Ra6. Gas chromatography-mass spectrometry was used for analysis of isotopic enrichment. At rest, 33% of total glucose flux was recycled. In exercise, total flux increased 300%, but so did glucose cycling, which means that there was no change in the percentage of flux recycled. In recovery, both total flux and the rate of recycling returned rapidly to the resting value. We therefore conclude that whereas total glucose production can respond extremely quickly to large changes in energy requirements caused by exercise, thereby enabling maintenance of a constant blood glucose concentration, glucose cycling does not have an important role in amplifying the control of net hepatic glucose flux through the glucose cycle.

Original languageEnglish
Pages (from-to)1815-1819
Number of pages5
JournalJournal of Applied Physiology
Issue number5
StatePublished - Jan 1 1990
Externally publishedYes


  • exercise
  • stable isotopes

Fingerprint Dive into the research topics of 'Role of the glucose cycle in control of net glucose flux in exercising humans'. Together they form a unique fingerprint.

  • Cite this