TY - JOUR
T1 - Role of the esophageal vagus neural pathway in ionizing irradiation-induced seizures in nitric oxide synthase-1 homologous recombinant negative NOS1 -/- mice
AU - Bernard, Mark E.
AU - Kim, Hyun
AU - Rwigema, Jean Claude
AU - Epperly, Michael W.
AU - Kelley, Eric E.
AU - Murdoch, Geoffery H.
AU - Dixon, Tracy
AU - Wang, Hong
AU - Greenberger, Joel S.
PY - 2011
Y1 - 2011
N2 - Aim: We sought to define the mechanism of total body irradiation (TBI)-induced seizures in NOS1-/- mice and amelioration by intra-esophageal manganese superoxide dismutase-plasmid liposomes (MnSOD-PL). Materials and Methods: We evaluated the role of vagus nerve pathways in irradiation-induced seizures using biochemical, physiologic, and histopathologic techniques. Results: Heterozygous NOS1+/- mice demonstrated radioresistance similar to wildtype C57BL/6NHsd mice (p=0.9269). Irradiation-induced lipid peroxidation in fetal brain cultures from NOS1 -/- or wild-type mice was reduced by MnSOD-PL. Right-sided vagotomy did not alter the TBI radiation response of wild-type or reverse the radiosensitivity of NOS1-/- mice. Excised esophagus from irradiated NOS1-/- mice demonstrated an increased histopathologic inflammatory response compared to C57BL/6NHsd mice. Conclusion: NOS1-/- mice represent a model system for dissecting the developmental abnormalities leading to esophageal-mediated TBI-induced seizures.
AB - Aim: We sought to define the mechanism of total body irradiation (TBI)-induced seizures in NOS1-/- mice and amelioration by intra-esophageal manganese superoxide dismutase-plasmid liposomes (MnSOD-PL). Materials and Methods: We evaluated the role of vagus nerve pathways in irradiation-induced seizures using biochemical, physiologic, and histopathologic techniques. Results: Heterozygous NOS1+/- mice demonstrated radioresistance similar to wildtype C57BL/6NHsd mice (p=0.9269). Irradiation-induced lipid peroxidation in fetal brain cultures from NOS1 -/- or wild-type mice was reduced by MnSOD-PL. Right-sided vagotomy did not alter the TBI radiation response of wild-type or reverse the radiosensitivity of NOS1-/- mice. Excised esophagus from irradiated NOS1-/- mice demonstrated an increased histopathologic inflammatory response compared to C57BL/6NHsd mice. Conclusion: NOS1-/- mice represent a model system for dissecting the developmental abnormalities leading to esophageal-mediated TBI-induced seizures.
KW - Irradiation-induced seizures
KW - Vagus nerve
UR - http://www.scopus.com/inward/record.url?scp=80755161409&partnerID=8YFLogxK
M3 - Article
C2 - 22021678
AN - SCOPUS:80755161409
SN - 0258-851X
VL - 25
SP - 861
EP - 869
JO - In Vivo
JF - In Vivo
IS - 6
ER -