Role of the esophageal vagus neural pathway in ionizing irradiation-induced seizures in nitric oxide synthase-1 homologous recombinant negative NOS1 -/- mice

Mark E. Bernard, Hyun Kim, Jean Claude Rwigema, Michael W. Epperly, Eric E. Kelley, Geoffery H. Murdoch, Tracy Dixon, Hong Wang, Joel S. Greenberger

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Aim: We sought to define the mechanism of total body irradiation (TBI)-induced seizures in NOS1-/- mice and amelioration by intra-esophageal manganese superoxide dismutase-plasmid liposomes (MnSOD-PL). Materials and Methods: We evaluated the role of vagus nerve pathways in irradiation-induced seizures using biochemical, physiologic, and histopathologic techniques. Results: Heterozygous NOS1+/- mice demonstrated radioresistance similar to wildtype C57BL/6NHsd mice (p=0.9269). Irradiation-induced lipid peroxidation in fetal brain cultures from NOS1 -/- or wild-type mice was reduced by MnSOD-PL. Right-sided vagotomy did not alter the TBI radiation response of wild-type or reverse the radiosensitivity of NOS1-/- mice. Excised esophagus from irradiated NOS1-/- mice demonstrated an increased histopathologic inflammatory response compared to C57BL/6NHsd mice. Conclusion: NOS1-/- mice represent a model system for dissecting the developmental abnormalities leading to esophageal-mediated TBI-induced seizures.

Original languageEnglish
Pages (from-to)861-869
Number of pages9
JournalIn Vivo
Volume25
Issue number6
StatePublished - 2011
Externally publishedYes

Keywords

  • Irradiation-induced seizures
  • Vagus nerve

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