Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient mice

S. L. Stanley, T. Zhang, D. Rubin, E. Li

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

Evidence from in vitro studies suggest that the Entamoeba histolytica cysteine proteinase plays a role in the tissue lysis and cytopathic effects seen in invasive amebiasis. We used affinity-purified antibodies against a recombinant E. histolytica cysteine proteinase to demonstrate that the proteinase is present extracellularly in amebic liver abscesses in mice with severe combined immunodeficiency (SCID mice). Treatment of E. histolytica trophozoites with specific cysteine proteinase inhibitor E-64 blocked or greatly reduced liver abscess formation at 48 h in SCID mice. Our study suggests an important role for a functional cysteine proteinase in amebic liver abscess formation.

Original languageEnglish
Pages (from-to)1587-1590
Number of pages4
JournalInfection and immunity
Volume63
Issue number4
StatePublished - Jan 1 1995

Fingerprint Dive into the research topics of 'Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient mice'. Together they form a unique fingerprint.

  • Cite this