Role of singlet oxygen in the degradation of hyaluronic acid

Usha P. Andley; Bireswar Chakrabarti

doi:10.1016/S0006-291X(83)80019-9

Role of singlet oxygen in the degradation of hyaluronic acid

Usha P. Andley, Bireswar Chakrabarti

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

To investigate the effect of singlet oxygen on the molecular properties of hyaluronic acid, the polymer was irradiated in the presence of a dye sensitizer for singlet oxygen. Viscosity and circular dichroism techniques were used to monitor these changes. The relative viscosity of the polymer solution decreased steadily with increasing duration of irradiation, indicating an apparent decrease in molecular weight of hyaluronic acid. Circular dichroism measurements of the irradiated sample, however, did not show any appreciable change in the secondary structure, but do suggest that the generated singlet oxygen changes the tertiary structure and that this change is followed by a minor depolymerization.

Original language	English
Pages (from-to)	894-901
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	115
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(83)80019-9
State	Published - Sep 30 1983

Access to Document

10.1016/S0006-291X(83)80019-9

Cite this

@article{167af7f2f8804af2b9967243f79e66d7,

title = "Role of singlet oxygen in the degradation of hyaluronic acid",

abstract = "To investigate the effect of singlet oxygen on the molecular properties of hyaluronic acid, the polymer was irradiated in the presence of a dye sensitizer for singlet oxygen. Viscosity and circular dichroism techniques were used to monitor these changes. The relative viscosity of the polymer solution decreased steadily with increasing duration of irradiation, indicating an apparent decrease in molecular weight of hyaluronic acid. Circular dichroism measurements of the irradiated sample, however, did not show any appreciable change in the secondary structure, but do suggest that the generated singlet oxygen changes the tertiary structure and that this change is followed by a minor depolymerization.",

author = "Andley, {Usha P.} and Bireswar Chakrabarti",

note = "Funding Information: ACKNOWLEDGMENTS: Supported by NIH grant EY01760, pr0ject EY04342, and Charles A. King Trust Fellowship.",

year = "1983",

month = sep,

day = "30",

doi = "10.1016/S0006-291X(83)80019-9",

language = "English",

volume = "115",

pages = "894--901",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

number = "3",

}

TY - JOUR

T1 - Role of singlet oxygen in the degradation of hyaluronic acid

AU - Andley, Usha P.

AU - Chakrabarti, Bireswar

N1 - Funding Information: ACKNOWLEDGMENTS: Supported by NIH grant EY01760, pr0ject EY04342, and Charles A. King Trust Fellowship.

PY - 1983/9/30

Y1 - 1983/9/30

N2 - To investigate the effect of singlet oxygen on the molecular properties of hyaluronic acid, the polymer was irradiated in the presence of a dye sensitizer for singlet oxygen. Viscosity and circular dichroism techniques were used to monitor these changes. The relative viscosity of the polymer solution decreased steadily with increasing duration of irradiation, indicating an apparent decrease in molecular weight of hyaluronic acid. Circular dichroism measurements of the irradiated sample, however, did not show any appreciable change in the secondary structure, but do suggest that the generated singlet oxygen changes the tertiary structure and that this change is followed by a minor depolymerization.

AB - To investigate the effect of singlet oxygen on the molecular properties of hyaluronic acid, the polymer was irradiated in the presence of a dye sensitizer for singlet oxygen. Viscosity and circular dichroism techniques were used to monitor these changes. The relative viscosity of the polymer solution decreased steadily with increasing duration of irradiation, indicating an apparent decrease in molecular weight of hyaluronic acid. Circular dichroism measurements of the irradiated sample, however, did not show any appreciable change in the secondary structure, but do suggest that the generated singlet oxygen changes the tertiary structure and that this change is followed by a minor depolymerization.

UR - http://www.scopus.com/inward/record.url?scp=0020618881&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(83)80019-9

DO - 10.1016/S0006-291X(83)80019-9

M3 - Article

C2 - 6626227

AN - SCOPUS:0020618881

SN - 0006-291X

VL - 115

SP - 894

EP - 901

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Role of singlet oxygen in the degradation of hyaluronic acid

Abstract

Access to Document

Other files and links

Fingerprint

Cite this