TY - JOUR
T1 - Role of nitric oxide in skeletal muscle blood flow at rest and during dynamic exercise in humans
AU - Hickner, R. C.
AU - Fisher, J. S.
AU - Ehsani, A. A.
AU - Kohrt, W. M.
PY - 1997/7
Y1 - 1997/7
N2 - The role of nitric oxide at rest and in the active hyperemic response within skeletal muscle was investigated in eight physically active men. Three microdialysis probes were inserted into the vastus lateralis of the quadriceps femoris muscle group in each subject. Microdialysis probes were perfused with a Ringer solution containing 5.0 mM ethanol, 2.5 mM glucose, and either 10 mg/ml of the nitric oxide synthase inhibitor N(G)-monomethyl- L-arginine (L-NMMA) and monoacetate salt, 30 mg/ml of the nitric oxide precursor L-arginine, or no additional substance (control probe). Subjects performed one-legged cycling exercise at work rates ranging from 25 to 100 W. Dialysate and perfusate ethanol concentrations were presented as the ratio of [ethanol](dialysate) to [ethanol](perfusate) (ethanol outflow-to-inflow ratio), an indicator that is inversely treated to blood flow. The ethanol outflow-to-inflow ratios at rest were 0.614 ± 0.032, 0.523 ± 0.023, and 0.578 ± 0.039 in the L-NMMA, L-arginine, and control probes, respectively. Calculated resting blood flows were therefore 8.7 ± 4.1, 20.5 ± 4.6, and 14.0 ± 4.7 ml · min-1 · 100 g-1 around the L-NMMA, L-arginine, and control probes, respectively. The ethanol outflow-to-inflow ratios were significantly higher at all exercise intensities in the L-NMMA probe than in the control and L-arginine probes, resulting in calculated blood flows of 195 ± 55,407 ± 47, and 352 ± 0 ml · min-1 · 100 g-1 at 25 W and 28 ± 5,602 ± 129, and 519 ± 113 ml · min-1 · 100 g-1 at 100 W around the L-NMMA, L-arginine, and control probes, respectively. Skeletal muscle blood flow was therefore reduced both at rest and during continuous, dynamic exercise by the action of L-NMMA, whereas blood flow was increased only at rest by L-arginine.
AB - The role of nitric oxide at rest and in the active hyperemic response within skeletal muscle was investigated in eight physically active men. Three microdialysis probes were inserted into the vastus lateralis of the quadriceps femoris muscle group in each subject. Microdialysis probes were perfused with a Ringer solution containing 5.0 mM ethanol, 2.5 mM glucose, and either 10 mg/ml of the nitric oxide synthase inhibitor N(G)-monomethyl- L-arginine (L-NMMA) and monoacetate salt, 30 mg/ml of the nitric oxide precursor L-arginine, or no additional substance (control probe). Subjects performed one-legged cycling exercise at work rates ranging from 25 to 100 W. Dialysate and perfusate ethanol concentrations were presented as the ratio of [ethanol](dialysate) to [ethanol](perfusate) (ethanol outflow-to-inflow ratio), an indicator that is inversely treated to blood flow. The ethanol outflow-to-inflow ratios at rest were 0.614 ± 0.032, 0.523 ± 0.023, and 0.578 ± 0.039 in the L-NMMA, L-arginine, and control probes, respectively. Calculated resting blood flows were therefore 8.7 ± 4.1, 20.5 ± 4.6, and 14.0 ± 4.7 ml · min-1 · 100 g-1 around the L-NMMA, L-arginine, and control probes, respectively. The ethanol outflow-to-inflow ratios were significantly higher at all exercise intensities in the L-NMMA probe than in the control and L-arginine probes, resulting in calculated blood flows of 195 ± 55,407 ± 47, and 352 ± 0 ml · min-1 · 100 g-1 at 25 W and 28 ± 5,602 ± 129, and 519 ± 113 ml · min-1 · 100 g-1 at 100 W around the L-NMMA, L-arginine, and control probes, respectively. Skeletal muscle blood flow was therefore reduced both at rest and during continuous, dynamic exercise by the action of L-NMMA, whereas blood flow was increased only at rest by L-arginine.
KW - Endothelium-derived relaxing factor
KW - L-arginine
KW - Microcirculation
KW - Microdialysis
KW - N(G)-monomethyl-L- arginine
UR - http://www.scopus.com/inward/record.url?scp=0030874355&partnerID=8YFLogxK
U2 - 10.1152/ajpheart.1997.273.1.h405
DO - 10.1152/ajpheart.1997.273.1.h405
M3 - Article
C2 - 9249515
AN - SCOPUS:0030874355
SN - 0363-6135
VL - 273
SP - H405-H410
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
IS - 1 42-1
ER -