Role of macrophages in delayed hypersensitivity. II. Effects of anti-macrophage antibody

Joseph D. Feldman; Emil R. Unanue

doi:10.1016/0008-8749(71)90047-5

Role of macrophages in delayed hypersensitivity. II. Effects of anti-macrophage antibody

Joseph D. Feldman, Emil R. Unanue

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Abstract

Rabbit antisera (AMS) to guinea pig macrophages and the γG fraction thereof (AMG) were specific for membrane antigens of monocytes/macrophages. The AMS and AMG bound to the surface of peritoneal macrophages, agglutinated these cells, and destroyed them. They were nontoxic for guinea pig lymphocytes. When injected intraperitoneally repeatedly into Hartley guinea pigs immunized with BSA in complete Freund's adjuvant, AMG did not affect the Arthus and delayed hypersensitivity (DH) skin reactions elicited by BSA and PPD at 6 and 24 hr. They did, however, diminish or eliminate the passive transfer of DH in irradiated strain 13 guinea pigs that were infused with lymph node and a limited number of bone marrow cells from immune donors. This effect was most likely the result of a destructive action against bone marrow elements or their progeny.

Original language	English
Pages (from-to)	275-282
Number of pages	8
Journal	Cellular Immunology
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/0008-8749(71)90047-5
State	Published - Jun 1971

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@article{047588754e88472e9970e602180a3fdb,

title = "Role of macrophages in delayed hypersensitivity. II. Effects of anti-macrophage antibody",

abstract = "Rabbit antisera (AMS) to guinea pig macrophages and the γG fraction thereof (AMG) were specific for membrane antigens of monocytes/macrophages. The AMS and AMG bound to the surface of peritoneal macrophages, agglutinated these cells, and destroyed them. They were nontoxic for guinea pig lymphocytes. When injected intraperitoneally repeatedly into Hartley guinea pigs immunized with BSA in complete Freund's adjuvant, AMG did not affect the Arthus and delayed hypersensitivity (DH) skin reactions elicited by BSA and PPD at 6 and 24 hr. They did, however, diminish or eliminate the passive transfer of DH in irradiated strain 13 guinea pigs that were infused with lymph node and a limited number of bone marrow cells from immune donors. This effect was most likely the result of a destructive action against bone marrow elements or their progeny.",

author = "Feldman, {Joseph D.} and Unanue, {Emil R.}",

note = "Funding Information: 2 Supported by a Senior Fellowship of the American Cancer Society, California Division. Present Address : Department of Pathology, Harvard Medical School, Boston, Mass. 0211 j. Funding Information: 1 This is Publication No. 468 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, Calif. This work was supported by a United States Public Health Grant AI-07007 and Atomic Energy Commission Contract AT (04-3) -779.",

year = "1971",

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doi = "10.1016/0008-8749(71)90047-5",

language = "English",

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journal = "Cellular Immunology",

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T1 - Role of macrophages in delayed hypersensitivity. II. Effects of anti-macrophage antibody

AU - Feldman, Joseph D.

AU - Unanue, Emil R.

N1 - Funding Information: 2 Supported by a Senior Fellowship of the American Cancer Society, California Division. Present Address : Department of Pathology, Harvard Medical School, Boston, Mass. 0211 j. Funding Information: 1 This is Publication No. 468 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, Calif. This work was supported by a United States Public Health Grant AI-07007 and Atomic Energy Commission Contract AT (04-3) -779.

PY - 1971/6

Y1 - 1971/6

N2 - Rabbit antisera (AMS) to guinea pig macrophages and the γG fraction thereof (AMG) were specific for membrane antigens of monocytes/macrophages. The AMS and AMG bound to the surface of peritoneal macrophages, agglutinated these cells, and destroyed them. They were nontoxic for guinea pig lymphocytes. When injected intraperitoneally repeatedly into Hartley guinea pigs immunized with BSA in complete Freund's adjuvant, AMG did not affect the Arthus and delayed hypersensitivity (DH) skin reactions elicited by BSA and PPD at 6 and 24 hr. They did, however, diminish or eliminate the passive transfer of DH in irradiated strain 13 guinea pigs that were infused with lymph node and a limited number of bone marrow cells from immune donors. This effect was most likely the result of a destructive action against bone marrow elements or their progeny.

AB - Rabbit antisera (AMS) to guinea pig macrophages and the γG fraction thereof (AMG) were specific for membrane antigens of monocytes/macrophages. The AMS and AMG bound to the surface of peritoneal macrophages, agglutinated these cells, and destroyed them. They were nontoxic for guinea pig lymphocytes. When injected intraperitoneally repeatedly into Hartley guinea pigs immunized with BSA in complete Freund's adjuvant, AMG did not affect the Arthus and delayed hypersensitivity (DH) skin reactions elicited by BSA and PPD at 6 and 24 hr. They did, however, diminish or eliminate the passive transfer of DH in irradiated strain 13 guinea pigs that were infused with lymph node and a limited number of bone marrow cells from immune donors. This effect was most likely the result of a destructive action against bone marrow elements or their progeny.

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U2 - 10.1016/0008-8749(71)90047-5

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JO - Cellular Immunology

JF - Cellular Immunology

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ER -

Role of macrophages in delayed hypersensitivity. II. Effects of anti-macrophage antibody

Abstract

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