Role of macrophages in delayed hypersensitivity. I. Induction with macrophage-bound antigen

Emil R. Unanue; Joseph D. Feldman

doi:10.1016/0008-8749(71)90046-3

Role of macrophages in delayed hypersensitivity. I. Induction with macrophage-bound antigen

Emil R. Unanue, Joseph D. Feldman

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Abstract

Macrophage-bound antigen inoculated into footpads of rats induced a state of delayed hypersensitivity (DH) equivalent to that induced by free antigen but less than that induced by antigen incorporated into complete Freund's adjuvant. Antigen bound to macrophages was more immunogenic than free antigen and elicited antibody responses in all injected rats. Macrophages injected intradermally into rats immunized with antigen in complete Freund's adjuvant excited an acute inflammatory reaction consisting of numerous polymorphonuclear leucocytes and abscesses. In the same animals free antigen produced typical DH lesions, and antigen bound to macrophages produced a mixture of polymorphonuclear and mononuclear cell infiltrates. Intradermal deposit of macrophages or macrophages with antigen increased the number of basophils in areas of inflammation. Basophils did not vary in number or distribution in DH lesions elicited by free antigen at 14 or 28 days after inoculation.

Original language	English
Pages (from-to)	269-274
Number of pages	6
Journal	Cellular Immunology
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/0008-8749(71)90046-3
State	Published - Jun 1971

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title = "Role of macrophages in delayed hypersensitivity. I. Induction with macrophage-bound antigen",

abstract = "Macrophage-bound antigen inoculated into footpads of rats induced a state of delayed hypersensitivity (DH) equivalent to that induced by free antigen but less than that induced by antigen incorporated into complete Freund's adjuvant. Antigen bound to macrophages was more immunogenic than free antigen and elicited antibody responses in all injected rats. Macrophages injected intradermally into rats immunized with antigen in complete Freund's adjuvant excited an acute inflammatory reaction consisting of numerous polymorphonuclear leucocytes and abscesses. In the same animals free antigen produced typical DH lesions, and antigen bound to macrophages produced a mixture of polymorphonuclear and mononuclear cell infiltrates. Intradermal deposit of macrophages or macrophages with antigen increased the number of basophils in areas of inflammation. Basophils did not vary in number or distribution in DH lesions elicited by free antigen at 14 or 28 days after inoculation.",

author = "Unanue, {Emil R.} and Feldman, {Joseph D.}",

note = "Funding Information: No. 467 from the Department of Experimental Pathology, Foundation, La Jolla, Calif. This work was supported by a United -41-07007 and Atomic Energy Commission Contract -4T (04-3) -779.",

year = "1971",

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doi = "10.1016/0008-8749(71)90046-3",

language = "English",

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journal = "Cellular Immunology",

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T1 - Role of macrophages in delayed hypersensitivity. I. Induction with macrophage-bound antigen

AU - Unanue, Emil R.

AU - Feldman, Joseph D.

N1 - Funding Information: No. 467 from the Department of Experimental Pathology, Foundation, La Jolla, Calif. This work was supported by a United -41-07007 and Atomic Energy Commission Contract -4T (04-3) -779.

PY - 1971/6

Y1 - 1971/6

N2 - Macrophage-bound antigen inoculated into footpads of rats induced a state of delayed hypersensitivity (DH) equivalent to that induced by free antigen but less than that induced by antigen incorporated into complete Freund's adjuvant. Antigen bound to macrophages was more immunogenic than free antigen and elicited antibody responses in all injected rats. Macrophages injected intradermally into rats immunized with antigen in complete Freund's adjuvant excited an acute inflammatory reaction consisting of numerous polymorphonuclear leucocytes and abscesses. In the same animals free antigen produced typical DH lesions, and antigen bound to macrophages produced a mixture of polymorphonuclear and mononuclear cell infiltrates. Intradermal deposit of macrophages or macrophages with antigen increased the number of basophils in areas of inflammation. Basophils did not vary in number or distribution in DH lesions elicited by free antigen at 14 or 28 days after inoculation.

AB - Macrophage-bound antigen inoculated into footpads of rats induced a state of delayed hypersensitivity (DH) equivalent to that induced by free antigen but less than that induced by antigen incorporated into complete Freund's adjuvant. Antigen bound to macrophages was more immunogenic than free antigen and elicited antibody responses in all injected rats. Macrophages injected intradermally into rats immunized with antigen in complete Freund's adjuvant excited an acute inflammatory reaction consisting of numerous polymorphonuclear leucocytes and abscesses. In the same animals free antigen produced typical DH lesions, and antigen bound to macrophages produced a mixture of polymorphonuclear and mononuclear cell infiltrates. Intradermal deposit of macrophages or macrophages with antigen increased the number of basophils in areas of inflammation. Basophils did not vary in number or distribution in DH lesions elicited by free antigen at 14 or 28 days after inoculation.

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JO - Cellular Immunology

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ER -

Role of macrophages in delayed hypersensitivity. I. Induction with macrophage-bound antigen

Abstract

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