TY - JOUR
T1 - Role of immunophenotyping in characterisation of blast crisis of chronic myeloid leukemia - A study of 25 cases
AU - Padmanabhan, Vijayalakshmi
AU - Varma, Neelam
AU - Dash, Sumitra
AU - Varma, Subhash
PY - 1996/1
Y1 - 1996/1
N2 - The blast cell populations of 25 patients of chronic myeloid leukemia in blast crisis (CML-BC) were studied for morphological, cytochemical and imrnunophenotypic features. The patients were divided into 6 broad groups based upon the pattern of surface marker positivity- myeloblastic, mixed myeloblastic, megakaryoblastic, mixed lineage, Iymphoid and undifferentiated blast crisis. Myeloperoxidase (MPO), Sudan Black B (SBB) and Chloroacetate esterase (CAE) stains showed 100% specificity for the myelomonocytic lineage but the sensitivity was low. Periodic acid Schiff (PAS) stain was neither specific nor sensitive for the lymphoid lineage. Immunophenotyping as compared to morphologic and cytochemical assessment, was seen to be most useful for assigning a lineage to leukemic cells in CML-BC.
AB - The blast cell populations of 25 patients of chronic myeloid leukemia in blast crisis (CML-BC) were studied for morphological, cytochemical and imrnunophenotypic features. The patients were divided into 6 broad groups based upon the pattern of surface marker positivity- myeloblastic, mixed myeloblastic, megakaryoblastic, mixed lineage, Iymphoid and undifferentiated blast crisis. Myeloperoxidase (MPO), Sudan Black B (SBB) and Chloroacetate esterase (CAE) stains showed 100% specificity for the myelomonocytic lineage but the sensitivity was low. Periodic acid Schiff (PAS) stain was neither specific nor sensitive for the lymphoid lineage. Immunophenotyping as compared to morphologic and cytochemical assessment, was seen to be most useful for assigning a lineage to leukemic cells in CML-BC.
KW - Blast crisis
KW - Chronic myeloid leukemia
KW - Immunophenotyping
KW - Lineage heterogeneity
UR - http://www.scopus.com/inward/record.url?scp=0029688663&partnerID=8YFLogxK
M3 - Article
C2 - 8755126
AN - SCOPUS:0029688663
VL - 39
SP - 13
EP - 17
JO - Indian Journal of Pathology and Microbiology
JF - Indian Journal of Pathology and Microbiology
SN - 0377-4929
IS - 1
ER -