Role of immunophenotyping in characterisation of blast crisis of chronic myeloid leukemia - A study of 25 cases

Vijayalakshmi Padmanabhan, Neelam Varma, Sumitra Dash, Subhash Varma

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3 Scopus citations

Abstract

The blast cell populations of 25 patients of chronic myeloid leukemia in blast crisis (CML-BC) were studied for morphological, cytochemical and imrnunophenotypic features. The patients were divided into 6 broad groups based upon the pattern of surface marker positivity- myeloblastic, mixed myeloblastic, megakaryoblastic, mixed lineage, Iymphoid and undifferentiated blast crisis. Myeloperoxidase (MPO), Sudan Black B (SBB) and Chloroacetate esterase (CAE) stains showed 100% specificity for the myelomonocytic lineage but the sensitivity was low. Periodic acid Schiff (PAS) stain was neither specific nor sensitive for the lymphoid lineage. Immunophenotyping as compared to morphologic and cytochemical assessment, was seen to be most useful for assigning a lineage to leukemic cells in CML-BC.

Original languageEnglish
Pages (from-to)13-17
Number of pages5
JournalIndian Journal of Pathology and Microbiology
Volume39
Issue number1
StatePublished - Jan 1996

Keywords

  • Blast crisis
  • Chronic myeloid leukemia
  • Immunophenotyping
  • Lineage heterogeneity

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