Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase

Kiran N. Mahajan, Beverly S. Mitchell

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

Terminal deoxynucleotidyl transferase (TdT, EC 2.7.7.31) adds nucleotides to DNA ends generated during V(D)J recombination that are subsequently processed by proteins involved in general doublestrand break (DSB) repair pathways. We report an association between TdT and a 55-kDa protein in lymphoid cells. This protein, identified as hPso4, is a homolog of the protein encoded by the PSO4/PRP19 gene in Saccharomyces cerevisiae that has pleiotropic functions in DNA recombination and error-prone repair. Purified hPso4 binds double-stranded DNA in a sequence-nonspecific manner but does not bind single-stranded DNA. hPso4 protein is induced 15- to 30-fold in cells by γ radiation and chemical mutagens but not by UV treatment. Loss of hPso4 expression induced by siRNA results in accumulation of DSBs, apoptosis, and decreased cell survival after DNA damage. We conclude that hPso4 plays a major and previously undefined role in mammalian DNA DSB repair.

Original languageEnglish
Pages (from-to)10746-10751
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number19
DOIs
StatePublished - Sep 16 2003

Keywords

  • Double-strand breaks
  • End joining

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