Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli

Gautam Das, Dinesh Kumar Thotala, Suman Kapoor, Sheelarani Karunanithi, Suman S. Thakur, N. Sadananda Singh, Umesh Varshney

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Translation initiation from the ribosomal P-site is the specialty of the initiator tRNAs (tRNAfMet). Presence of the three consecutive G-C base pairs (G29-C41, G30-C40 and G31-C39) in their anticodon stems, a highly conserved feature of the initiator tRNAs across the three kingdoms of life, has been implicated in their preferential binding to the P-site. How this feature is exploited by ribosomes has remained unclear. Using a genetic screen, we have isolated an Escherichia coli strain, carrying a G122D mutation in folD, which allows initiation with the tRNAfMet containing mutations in one, two or all the three G-C base pairs. The strain shows a severe deficiency of methionine and S-adenosylmethionine, and lacks nucleoside methylations in rRNA. Targeted mutations in the methyltransferase genes have revealed a connection between the rRNA modifications and the fundamental process of the initiator tRNA selection by the ribosome.

Original languageEnglish
Pages (from-to)840-851
Number of pages12
JournalEMBO Journal
Volume27
Issue number6
DOIs
StatePublished - Mar 19 2008
Externally publishedYes

Keywords

  • 5,10 methylene tetrahydrofolate dehydrogenase
  • Methyltransferase
  • Modified nucleoside
  • S-adenosyl-L-methionine
  • folD

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    Das, G., Thotala, D. K., Kapoor, S., Karunanithi, S., Thakur, S. S., Singh, N. S., & Varshney, U. (2008). Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli. EMBO Journal, 27(6), 840-851. https://doi.org/10.1038/emboj.2008.20