Role for the Salmonella flavohemoglobin in protection from nitric oxide

Michael J. Crawford, Daniel E. Goldberg

Research output: Contribution to journalArticle

155 Scopus citations


Hemoglobin homologs are being identified in an expanding number of unicellular prokaryotic and eukaryotic organisms. Many of these hemoglobins are two-domain proteins t hat possess a flavin-containing reductase in their C terminus. Determination of a function for these flavohemoglobins has been elusive. A Salmonella typhimurium strain harboring a deletion in the flavohemoglobin gene shows no difference in growth under oxidative stress conditions but displays an increased sensitivity to acidified nitrite and S- nitrosothiols, both of which produce nitric oxide. The effect is seen aerobically or anaerobically, indicating that oxygen is not required for flavohemoglobin function. These results suggest a role for the bacterial flavohemoglobins that is independent of oxygen metabolism and provide evidence for a bacterial route of protection from nitric oxide that is distinct from oxidative stress responses.

Original languageEnglish
Pages (from-to)12543-12547
Number of pages5
JournalJournal of Biological Chemistry
Issue number20
StatePublished - May 15 1998

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