TY - JOUR
T1 - Role for the Salmonella flavohemoglobin in protection from nitric oxide
AU - Crawford, Michael J.
AU - Goldberg, Daniel E.
PY - 1998/5/15
Y1 - 1998/5/15
N2 - Hemoglobin homologs are being identified in an expanding number of unicellular prokaryotic and eukaryotic organisms. Many of these hemoglobins are two-domain proteins t hat possess a flavin-containing reductase in their C terminus. Determination of a function for these flavohemoglobins has been elusive. A Salmonella typhimurium strain harboring a deletion in the flavohemoglobin gene shows no difference in growth under oxidative stress conditions but displays an increased sensitivity to acidified nitrite and S- nitrosothiols, both of which produce nitric oxide. The effect is seen aerobically or anaerobically, indicating that oxygen is not required for flavohemoglobin function. These results suggest a role for the bacterial flavohemoglobins that is independent of oxygen metabolism and provide evidence for a bacterial route of protection from nitric oxide that is distinct from oxidative stress responses.
AB - Hemoglobin homologs are being identified in an expanding number of unicellular prokaryotic and eukaryotic organisms. Many of these hemoglobins are two-domain proteins t hat possess a flavin-containing reductase in their C terminus. Determination of a function for these flavohemoglobins has been elusive. A Salmonella typhimurium strain harboring a deletion in the flavohemoglobin gene shows no difference in growth under oxidative stress conditions but displays an increased sensitivity to acidified nitrite and S- nitrosothiols, both of which produce nitric oxide. The effect is seen aerobically or anaerobically, indicating that oxygen is not required for flavohemoglobin function. These results suggest a role for the bacterial flavohemoglobins that is independent of oxygen metabolism and provide evidence for a bacterial route of protection from nitric oxide that is distinct from oxidative stress responses.
UR - http://www.scopus.com/inward/record.url?scp=0032524650&partnerID=8YFLogxK
U2 - 10.1074/jbc.273.20.12543
DO - 10.1074/jbc.273.20.12543
M3 - Article
C2 - 9575213
AN - SCOPUS:0032524650
SN - 0021-9258
VL - 273
SP - 12543
EP - 12547
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 20
ER -