Role for plastin in host defense distinguishes integrin signaling from cell adhesion and spreading

Hua Chen; Attila Mocsai; Hong Zhang; Rong Xian Ding; J. Hiroshi Morisaki; Michael White; Jacob M. Rothfork; Patrick Heiser; Emma Colucci-Guyon; Clifford A. Lowell; Hattie D. Gresham; Paul M. Allen; Eric J. Brown

doi:10.1016/S1074-7613(03)00172-9

Role for plastin in host defense distinguishes integrin signaling from cell adhesion and spreading

Hua Chen, Attila Mocsai, Hong Zhang, Rong Xian Ding, J. Hiroshi Morisaki, Michael White, Jacob M. Rothfork, Patrick Heiser, Emma Colucci-Guyon, Clifford A. Lowell, Hattie D. Gresham, Paul M. Allen, Eric J. Brown

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90 Scopus citations

Abstract

Integrin ligation activates both cell adhesion and signal transduction, in part through reorganization of the actin cytoskeleton. Plastins (also known as fimbrins) are actin-crosslinking proteins of the cortical cytoskeleton present in all cells and conserved from yeast to mammals. Here we show that plastin-deficient polymorphonuclear neutrophils (PMN) are deficient in killing the bacterial pathogen Staphylococcus aureus in vivo and in vitro, despite normal phagocytosis. Like integrin β2-deficient PMN, plastin-deficient PMN cannot generate an adhesion-dependent respiratory burst, because of markedly diminished integrin-dependent syk activation. Unlike β2^-/- PMN, plastin-deficient PMN adhere and spread normally. Deficiency of plastin thus separates the classical integrin receptor functions of adhesion and spreading from intracellular signal transduction.

Original language	English
Pages (from-to)	95-104
Number of pages	10
Journal	Immunity
Volume	19
Issue number	1
DOIs	https://doi.org/10.1016/S1074-7613(03)00172-9
State	Published - Jul 1 2003

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@article{7cbebaa7217847f3bd74ab6efc18670d,

title = "Role for plastin in host defense distinguishes integrin signaling from cell adhesion and spreading",

abstract = "Integrin ligation activates both cell adhesion and signal transduction, in part through reorganization of the actin cytoskeleton. Plastins (also known as fimbrins) are actin-crosslinking proteins of the cortical cytoskeleton present in all cells and conserved from yeast to mammals. Here we show that plastin-deficient polymorphonuclear neutrophils (PMN) are deficient in killing the bacterial pathogen Staphylococcus aureus in vivo and in vitro, despite normal phagocytosis. Like integrin β2-deficient PMN, plastin-deficient PMN cannot generate an adhesion-dependent respiratory burst, because of markedly diminished integrin-dependent syk activation. Unlike β2-/- PMN, plastin-deficient PMN adhere and spread normally. Deficiency of plastin thus separates the classical integrin receptor functions of adhesion and spreading from intracellular signal transduction.",

author = "Hua Chen and Attila Mocsai and Hong Zhang and Ding, {Rong Xian} and Morisaki, {J. Hiroshi} and Michael White and Rothfork, {Jacob M.} and Patrick Heiser and Emma Colucci-Guyon and Lowell, {Clifford A.} and Gresham, {Hattie D.} and Allen, {Paul M.} and Brown, {Eric J.}",

note = "Funding Information: The authors thank Bill Nauseef (U. Iowa), Henry Bourne (UCSF), and Dean Sheppard (UCSF) for suggestions and critical review of the manuscript, Y.W. Kan for ES cells, and Dr. Sheppard for the modified tenascin. Supported by grants to E.J.B., P.M.A., H.D.G., and C.A.L. from NIH.",

year = "2003",

month = jul,

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doi = "10.1016/S1074-7613(03)00172-9",

language = "English",

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pages = "95--104",

journal = "Immunity",

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TY - JOUR

T1 - Role for plastin in host defense distinguishes integrin signaling from cell adhesion and spreading

AU - Chen, Hua

AU - Mocsai, Attila

AU - Zhang, Hong

AU - Ding, Rong Xian

AU - Morisaki, J. Hiroshi

AU - White, Michael

AU - Rothfork, Jacob M.

AU - Heiser, Patrick

AU - Colucci-Guyon, Emma

AU - Lowell, Clifford A.

AU - Gresham, Hattie D.

AU - Allen, Paul M.

AU - Brown, Eric J.

N1 - Funding Information: The authors thank Bill Nauseef (U. Iowa), Henry Bourne (UCSF), and Dean Sheppard (UCSF) for suggestions and critical review of the manuscript, Y.W. Kan for ES cells, and Dr. Sheppard for the modified tenascin. Supported by grants to E.J.B., P.M.A., H.D.G., and C.A.L. from NIH.

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Integrin ligation activates both cell adhesion and signal transduction, in part through reorganization of the actin cytoskeleton. Plastins (also known as fimbrins) are actin-crosslinking proteins of the cortical cytoskeleton present in all cells and conserved from yeast to mammals. Here we show that plastin-deficient polymorphonuclear neutrophils (PMN) are deficient in killing the bacterial pathogen Staphylococcus aureus in vivo and in vitro, despite normal phagocytosis. Like integrin β2-deficient PMN, plastin-deficient PMN cannot generate an adhesion-dependent respiratory burst, because of markedly diminished integrin-dependent syk activation. Unlike β2-/- PMN, plastin-deficient PMN adhere and spread normally. Deficiency of plastin thus separates the classical integrin receptor functions of adhesion and spreading from intracellular signal transduction.

AB - Integrin ligation activates both cell adhesion and signal transduction, in part through reorganization of the actin cytoskeleton. Plastins (also known as fimbrins) are actin-crosslinking proteins of the cortical cytoskeleton present in all cells and conserved from yeast to mammals. Here we show that plastin-deficient polymorphonuclear neutrophils (PMN) are deficient in killing the bacterial pathogen Staphylococcus aureus in vivo and in vitro, despite normal phagocytosis. Like integrin β2-deficient PMN, plastin-deficient PMN cannot generate an adhesion-dependent respiratory burst, because of markedly diminished integrin-dependent syk activation. Unlike β2-/- PMN, plastin-deficient PMN adhere and spread normally. Deficiency of plastin thus separates the classical integrin receptor functions of adhesion and spreading from intracellular signal transduction.

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U2 - 10.1016/S1074-7613(03)00172-9

DO - 10.1016/S1074-7613(03)00172-9

M3 - Article

C2 - 12871642

AN - SCOPUS:0038106595

SN - 1074-7613

VL - 19

SP - 95

EP - 104

JO - Immunity

JF - Immunity

IS - 1

ER -

Role for plastin in host defense distinguishes integrin signaling from cell adhesion and spreading

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