Robust fluorescent response of micropatterned multilayered films

Srikanth Singamaneni; Chaoyang Jiang; Emily Merrick; Dinesh Kommireddy; Vladimir V. Tsukruk

doi:10.1080/00222340601036728

Robust fluorescent response of micropatterned multilayered films

Srikanth Singamaneni, Chaoyang Jiang, Emily Merrick, Dinesh Kommireddy, Vladimir V. Tsukruk

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate that photobleaching can be significantly suppressed in fluorescent-labeled polyelectrolyte layer-by-layer (LbL) films by efficient spatial isolation of the dye molecules using multilayered architecture. The films exhibited excellent temporal stability under high excitation power where the bulk films suffered a significant photobleaching. Quenching of the fluorescence caused by the resonant energy transfer between the dye molecules and gold nanoparticles exhibited a weak distance dependence with significant quenching extending beyond 20nm distance. The robust, freely suspended, fluorescent LbL films exhibited stable fluorescence response under deformation which can make them attractive for sensitive fluoroimmunoassays.

Original language	English
Pages (from-to)	7-19
Number of pages	13
Journal	Journal of Macromolecular Science, Part B: Physics
Volume	46 B
Issue number	1
DOIs	https://doi.org/10.1080/00222340601036728
State	Published - Jan 2007

Keywords

Atomic force microscopy
Energy transfer
Fluorescence
LbL films
Photobleaching

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10.1080/00222340601036728

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title = "Robust fluorescent response of micropatterned multilayered films",

abstract = "We demonstrate that photobleaching can be significantly suppressed in fluorescent-labeled polyelectrolyte layer-by-layer (LbL) films by efficient spatial isolation of the dye molecules using multilayered architecture. The films exhibited excellent temporal stability under high excitation power where the bulk films suffered a significant photobleaching. Quenching of the fluorescence caused by the resonant energy transfer between the dye molecules and gold nanoparticles exhibited a weak distance dependence with significant quenching extending beyond 20nm distance. The robust, freely suspended, fluorescent LbL films exhibited stable fluorescence response under deformation which can make them attractive for sensitive fluoroimmunoassays.",

keywords = "Atomic force microscopy, Energy transfer, Fluorescence, LbL films, Photobleaching",

author = "Srikanth Singamaneni and Chaoyang Jiang and Emily Merrick and Dinesh Kommireddy and Tsukruk, {Vladimir V.}",

note = "Funding Information: The authors thank Prof. Y. Lvov for his suggestions in labeling the polyelectrolytes. The authors thank H. Ko for invaluable help with micropatterning and M.E. McConney for useful discussions. This work was supported by the AFOSR, FA9550-05-1-0209 and NSF-CTS-0506832 Grants.",

year = "2007",

month = jan,

doi = "10.1080/00222340601036728",

language = "English",

volume = "46 B",

pages = "7--19",

journal = "Journal of Macromolecular Science, Part B: Physics",

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TY - JOUR

T1 - Robust fluorescent response of micropatterned multilayered films

AU - Singamaneni, Srikanth

AU - Jiang, Chaoyang

AU - Merrick, Emily

AU - Kommireddy, Dinesh

AU - Tsukruk, Vladimir V.

N1 - Funding Information: The authors thank Prof. Y. Lvov for his suggestions in labeling the polyelectrolytes. The authors thank H. Ko for invaluable help with micropatterning and M.E. McConney for useful discussions. This work was supported by the AFOSR, FA9550-05-1-0209 and NSF-CTS-0506832 Grants.

PY - 2007/1

Y1 - 2007/1

N2 - We demonstrate that photobleaching can be significantly suppressed in fluorescent-labeled polyelectrolyte layer-by-layer (LbL) films by efficient spatial isolation of the dye molecules using multilayered architecture. The films exhibited excellent temporal stability under high excitation power where the bulk films suffered a significant photobleaching. Quenching of the fluorescence caused by the resonant energy transfer between the dye molecules and gold nanoparticles exhibited a weak distance dependence with significant quenching extending beyond 20nm distance. The robust, freely suspended, fluorescent LbL films exhibited stable fluorescence response under deformation which can make them attractive for sensitive fluoroimmunoassays.

AB - We demonstrate that photobleaching can be significantly suppressed in fluorescent-labeled polyelectrolyte layer-by-layer (LbL) films by efficient spatial isolation of the dye molecules using multilayered architecture. The films exhibited excellent temporal stability under high excitation power where the bulk films suffered a significant photobleaching. Quenching of the fluorescence caused by the resonant energy transfer between the dye molecules and gold nanoparticles exhibited a weak distance dependence with significant quenching extending beyond 20nm distance. The robust, freely suspended, fluorescent LbL films exhibited stable fluorescence response under deformation which can make them attractive for sensitive fluoroimmunoassays.

KW - Atomic force microscopy

KW - Energy transfer

KW - Fluorescence

KW - LbL films

KW - Photobleaching

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U2 - 10.1080/00222340601036728

DO - 10.1080/00222340601036728

M3 - Article

AN - SCOPUS:33947419869

SN - 0022-2348

VL - 46 B

SP - 7

EP - 19

JO - Journal of Macromolecular Science, Part B: Physics

JF - Journal of Macromolecular Science, Part B: Physics

IS - 1

ER -

Robust fluorescent response of micropatterned multilayered films

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Keywords

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