Robust deep brain stimulation functional MRI procedures in rats and mice using an MR-compatible tungsten microwire electrode

Hsin Yi Lai, Daniel L. Albaugh, Yu Chieh Jill Kao, John R. Younce, Yen Yu Ian Shih

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Purpose: To develop a series of robust and readily adoptable protocols for the application of deep brain stimulation (DBS)-functional MRI (fMRI) in rodents. Methods: DBS-fMRI procedures were conducted in rat and mouse under varying anesthetic conditions (isoflurane in rat and mouse, α-chloralose in rat). A homemade two-channel tungsten microwire electrode was used to minimize magnetic susceptibility artifacts, and was targeted to the ventral poster-omedial (VPM) thalamus for DBS-fMRI scanning procedures. Results: Compared with a commercially available MR-compatible electrode, the tungsten microwire generated greatly reduced magnetic-susceptibility artifacts. In the rat, VPM-DBS using the microwire electrode resulted in robust positive blood-oxygen- level-dependent signal changes in somatosensory cortex that were relatively independent of anesthetic type. In the mouse, VPM-DBS similarly generated large, positive neurovascular responses in somatosensory cortex that were detected using cerebral blood volume measurements. Conclusion: Collectively, this work describes reasonable and easily adoptable procedures for conducting DBS-fMRI studies in rodent models. The protocols developed herein may be extended to study DBS effects under numerous experimental conditions and at varying stimulation targets.

Original languageEnglish
Pages (from-to)1246-1251
Number of pages6
JournalMagnetic resonance in medicine
Volume73
Issue number3
DOIs
StatePublished - Mar 1 2015

Keywords

  • Deep brain stimulation
  • Mouse
  • Rat
  • Tungsten electrode
  • fMRI

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