Abstract
TRA-1, a member of the GLI family of transcription factors, is required for C. elegans female development. We find that TRA-1 has a sex-specific distribution consistent with its role in female development: nuclear TRA-1 is higher in hermaphrodite intestines and in specific germline regions than in males. TRA-1 patterns rely on nuclear export since treatment with leptomycin B, a CRM1-dependent export inhibitor, increases nuclear TRA-1 in males. TRA-1 export requires TRA-1 binding to the tra-2 3′ untranslated region (3′ UTR), as disruption of binding increases nuclear TRA-1 and female development. Our data are consistent with coexport of a TRA-1/tra-2 mRNA complex reducing TRA-1 nuclear activity, and identify an interesting RNA-based mechanism for controlling transcriptional activity and cell fate determination.
Original language | English |
---|---|
Pages (from-to) | 539-551 |
Number of pages | 13 |
Journal | Developmental cell |
Volume | 1 |
Issue number | 4 |
DOIs | |
State | Published - Oct 2001 |