RNA-Regulated TRA-1 Nuclear Export Controls Sexual Fate

S. P. Segal, L. E. Graves, J. Verheyden, E. B. Goodwin

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

TRA-1, a member of the GLI family of transcription factors, is required for C. elegans female development. We find that TRA-1 has a sex-specific distribution consistent with its role in female development: nuclear TRA-1 is higher in hermaphrodite intestines and in specific germline regions than in males. TRA-1 patterns rely on nuclear export since treatment with leptomycin B, a CRM1-dependent export inhibitor, increases nuclear TRA-1 in males. TRA-1 export requires TRA-1 binding to the tra-2 3′ untranslated region (3′ UTR), as disruption of binding increases nuclear TRA-1 and female development. Our data are consistent with coexport of a TRA-1/tra-2 mRNA complex reducing TRA-1 nuclear activity, and identify an interesting RNA-based mechanism for controlling transcriptional activity and cell fate determination.

Original languageEnglish
Pages (from-to)539-551
Number of pages13
JournalDevelopmental cell
Volume1
Issue number4
DOIs
StatePublished - Oct 2001
Externally publishedYes

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