RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation

Jordina Guillén-Boixet, Andrii Kopach, Alex S. Holehouse, Sina Wittmann, Marcus Jahnel, Raimund Schlüßler, Kyoohyun Kim, Irmela R.E.A. Trussina, Jie Wang, Daniel Mateju, Ina Poser, Shovamayee Maharana, Martine Ruer-Gruß, Doris Richter, Xiaojie Zhang, Young Tae Chang, Jochen Guck, Alf Honigmann, Julia Mahamid, Anthony A. HymanRohit V. Pappu, Simon Alberti, Titus M. Franzmann

Research output: Contribution to journalArticlepeer-review

197 Scopus citations

Abstract

Reconstitution of stress granule assembly reveals an autoinhibitory conformation of G3BP that is alleviated by RNA binding, demonstrating how this central node of the stress granule network phase-separates in response to rising cellular RNA concentrations.

Original languageEnglish
Pages (from-to)346-361.e17
JournalCell
Volume181
Issue number2
DOIs
StatePublished - Apr 16 2020

Keywords

  • G3BP
  • Neurodegenerative disease
  • RNP granules
  • liquid-to-solid transition
  • phase separation
  • stress granules
  • stress response

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