RNA editing in wheat mitochondria proceeds by a deamination mechanism

Valérie Blanc, Simón Litvak, Alejandro Araya

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Most if not all mitochondrial messenger RNAs from seed plants undergo a post-transcriptional modification (RNA editing) involving the conversion of some cytidine residues to uridine. Using a molecular hybridization approach, an in vitro RNA editing system, able to faithfully reproduce the in vivo observed C to U changes of subunit 9 (atp9) of wheat mitochondrial ATP synthase mRNA, has been described [Araya et al. (1992) Proc. Natl. Acad. Sci. USA 89, 1040-1044]. In this work we extend these studies to better understand the biochemical mechanism of this process. RNA editing was analysed by P1 nuclease digestion of the reaction product followed by thin layer chromatography. Experiments performed with unedited [3H]RNA labelled on the base and with unedited [32P]RNA labelled at the α-phosphate of cytidine residues, indicate that plant mitochondrial RNA editing operates through a deamination mechanism.

Original languageEnglish
Pages (from-to)56-60
Number of pages5
JournalFEBS Letters
Volume373
Issue number1
DOIs
StatePublished - Oct 2 1995

Keywords

  • ATPase subunit 9
  • Plant mitochondrion
  • RNA editing
  • Triticum aestivum

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