Ribonuclease A cleavage combined with the polymerase chain reaction for detection of the Z mutation of the alpha-1-antitrypsin gene.

Medicine & Life Sciences

• Pancreatic Ribonuclease 100%
• alpha 1-Antitrypsin 92%
• Exons 58%
• Polymerase Chain Reaction 41%
• Antisense Elements (Genetics) 41%
• Mutation 39%
• RNA Probes 37%
• Antisense RNA 33%
• DNA 29%
• Genes 28%
• 3' Flanking Region 12%
• alpha 1-Antitrypsin Deficiency 12%
• Alleles 12%
• RNA 12%
• 5' Flanking Region 10%
• Ribonucleases 9%
• Emphysema 9%
• DNA-Directed DNA Polymerase 9%
• Homozygote 9%
• Heterozygote 9%
• Autoradiography 9%
• Polyacrylamide Gel Electrophoresis 8%
• Organism Cloning 8%
• Digestion 7%
• Liver Diseases 7%
• Phenotype 5%

Chemical Compounds

• Chain Reaction 68%
• Mutation 59%
• RNA Probe 54%
• Cleavage 47%
• Sequencing Method 17%
• Probe 14%
• Polyacrylamide Gel Electrophoresis 12%
• Substitution Reaction 7%
• Length 6%