TY - JOUR
T1 - RhCG is downregulated in oesophageal squamous cell carcinomas, but expressed in multiple squamous epithelia
AU - Chen, Bao Sheng
AU - Xu, Zhi Xiong
AU - Xu, Xin
AU - Cai, Yan
AU - Han, Ya Ling
AU - Wang, Jie
AU - Xia, Shu Hua
AU - Hu, Hai
AU - Wei, Fang
AU - Wu, Min
AU - Wang, Ming Rong
N1 - Funding Information:
This work was supported by the Chinese Hi-Tech R&D Program Grant (2001AA221151), State Key Basic Research Grant of China (G1988051205) and National Natural Science Foundation (30125026).
PY - 2002/9
Y1 - 2002/9
N2 - To better understand the molecular events underlying the development of oesophageal cancer, we have isolated the genes dysregulated in primary oesophageal cancer tissues using a modified differential display polymerase chain reaction (DD-PCR). In the present study, a gene designated C15orf6 was identified. The C15orf6 gene, encompassing 25 kb, is composed of 11 exons with a mRNA of 1948 bp. Database searching showed that C15orf6 was 100% homologous to the Rh type C-glycoprotein (RhCG) with the same open reading frame, but 16 bp longer than RhCG at the 5′-end. The gene was highly expressed in human oesophagus, cervix, oral cavity, skin and kidney, but undetectable in the other 14 adult normal tissues examined. Northern blot, RT-PCR and western blot analysis showed that RhCG/C15orf6 was frequently lost or dramatically reduced in primary oesophageal cancer tissues (30/34) compared with the corresponding normal oesophageal mucosa. Three oesophageal-cancer cell lines tested lacked RhCG/C15orf6 expression. Immunohistochemistry revealed that in normal oesophageal tissues, RhCG/C15orf6 was mainly expressed in the plasma membrane of the epithelial cells. In addition, Rh-associated glycoprotein (RhAG) expression was also commonly silenced in both oesophageal cancer cell lines (2/3) and primary oesophageal cancer tissues (11/13). To our knowledge, this is the first time that RhAG expression has been seen in oesophageal epithelium and extends the functional role of the RhAG protein beyond the erythrocyte. These data suggest that inactivation of RhCG/C15orf6 and RhAG occurs frequently during the development of human oesophageal cancer.
AB - To better understand the molecular events underlying the development of oesophageal cancer, we have isolated the genes dysregulated in primary oesophageal cancer tissues using a modified differential display polymerase chain reaction (DD-PCR). In the present study, a gene designated C15orf6 was identified. The C15orf6 gene, encompassing 25 kb, is composed of 11 exons with a mRNA of 1948 bp. Database searching showed that C15orf6 was 100% homologous to the Rh type C-glycoprotein (RhCG) with the same open reading frame, but 16 bp longer than RhCG at the 5′-end. The gene was highly expressed in human oesophagus, cervix, oral cavity, skin and kidney, but undetectable in the other 14 adult normal tissues examined. Northern blot, RT-PCR and western blot analysis showed that RhCG/C15orf6 was frequently lost or dramatically reduced in primary oesophageal cancer tissues (30/34) compared with the corresponding normal oesophageal mucosa. Three oesophageal-cancer cell lines tested lacked RhCG/C15orf6 expression. Immunohistochemistry revealed that in normal oesophageal tissues, RhCG/C15orf6 was mainly expressed in the plasma membrane of the epithelial cells. In addition, Rh-associated glycoprotein (RhAG) expression was also commonly silenced in both oesophageal cancer cell lines (2/3) and primary oesophageal cancer tissues (11/13). To our knowledge, this is the first time that RhAG expression has been seen in oesophageal epithelium and extends the functional role of the RhAG protein beyond the erythrocyte. These data suggest that inactivation of RhCG/C15orf6 and RhAG occurs frequently during the development of human oesophageal cancer.
KW - Downregulation
KW - Oesophageal squamous cell carcinoma
KW - RhCG
KW - Squamous epithelia
UR - http://www.scopus.com/inward/record.url?scp=0036710210&partnerID=8YFLogxK
U2 - 10.1016/S0959-8049(02)00190-9
DO - 10.1016/S0959-8049(02)00190-9
M3 - Article
C2 - 12204676
AN - SCOPUS:0036710210
SN - 0959-8049
VL - 38
SP - 1927
EP - 1936
JO - European Journal of Cancer
JF - European Journal of Cancer
IS - 14
ER -