RGS proteins inhibit Xwnt-8 signaling in Xenopus embryonic development

Chunlai Wu, Qingyi Zeng, Kendall J. Blumer, Anthony J. Muslin

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

RGS family members are GTPase activating proteins (GAPs) that antagonize signaling by heterotrimeric G proteins. Injection of Xenopus embryos with RNA encoding rat RGS4 (rRGS4), a GAP for G(i) and G(q), resulted in shortened trunks and decreased skeletal muscle. This phenotype is nearly identical to the effect of injection of either frzb or dominant negative Xwnt-8. Injection of human RGS2, which selectively deactivates G(q), had similar effects. rRGS4 inhibited the ability of early Xwnt-8 but not Xdsh misexpression to cause axis duplication. This effect is distinct from axin family members that contain RGS-like domains but act downstream of Xdsh. We identified two Xenopus RGS4 homologs, one of which, Xrgs4a, was expressed as a Spemann organizer component. Injection of Xenopus embryos with Xrgs4a also resulted in shortened trunks and decreased skeletal muscle. These results suggest that RGS proteins modulate Xwnt-8 signaling by attenuating the function of a G protein.

Original languageEnglish
Pages (from-to)2773-2784
Number of pages12
JournalDevelopment
Volume127
Issue number13
StatePublished - Jul 1 2000

Keywords

  • G protein
  • GAP
  • RGS
  • Signal transduction
  • Xenopus
  • Xwnt-8

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