Reversal of impaired wound healing in irradiated rats by platelet-derived growth faetor-BB

Thomas A. Mustoe, James Purdy, Peggy Gramates, Thomas F. Deuel, Arlen Thomason, Glenn F. Pierce

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

This study examined the potential influence of platelet-derived growth factor-BB homodimers (PDGF-BB) on surgical incisions in irradiated animals with depressed wound healing. Rats were irradiated with either 800 rods total body or 2,500 rads surface irradiation. Parallel dorsal skin incisions were made 2 days later, and PDGF-BB was applied topically a single time to one of two incisions. In total body-irradiated rats, bone marrow-derived elements were severely depressed, wound macrophages were virtually eliminated, and PDGF-BB treatment was ineffective. However, in surface-irradiated rats, PDGF-BB treatment recruited macrophages into wounds and partially reversed impaired healing on day 7 (p < 0.005) and day 12 (p < 0.001). PDGF-BB-treated wounds were 50 percent stronger than the paired control wounds. The results suggest PDGF requires bone marrow-derived cells, likely wound macrophages, for activity and that it may be useful as a topical agent in postirradiation surgical incisions.

Original languageEnglish
Pages (from-to)345-350
Number of pages6
JournalThe American Journal of Surgery
Volume158
Issue number4
DOIs
StatePublished - Oct 1989

Fingerprint

Dive into the research topics of 'Reversal of impaired wound healing in irradiated rats by platelet-derived growth faetor-BB'. Together they form a unique fingerprint.

Cite this