TY - JOUR
T1 - Reversal of impaired wound healing in irradiated rats by platelet-derived growth faetor-BB
AU - Mustoe, Thomas A.
AU - Purdy, James
AU - Gramates, Peggy
AU - Deuel, Thomas F.
AU - Thomason, Arlen
AU - Pierce, Glenn F.
N1 - Funding Information:
&in the Division of plastic Surgery, D&partment of Surgery; Physics Section, biv&ion of Rkliation Oncology, Maliinckrodt Department of Radiw, and the Departmcntp of Meditie and Biological Chemistry, Wa&bgton University Medical Center, St. Louis, Missouri and the Departments of h@ecuhu and Celhdar Biology and Experimental Pathology, Amgen, Thousand Oaks, California. Supported in part by National Institutes, of Health Grant GM41303 and gmnts from Am-gen, Tho&and Oaks, Calif-and the Division tif Plastic Surgery, Bamea HoEpiti, st. Louis, Mii.
PY - 1989/10
Y1 - 1989/10
N2 - This study examined the potential influence of platelet-derived growth factor-BB homodimers (PDGF-BB) on surgical incisions in irradiated animals with depressed wound healing. Rats were irradiated with either 800 rods total body or 2,500 rads surface irradiation. Parallel dorsal skin incisions were made 2 days later, and PDGF-BB was applied topically a single time to one of two incisions. In total body-irradiated rats, bone marrow-derived elements were severely depressed, wound macrophages were virtually eliminated, and PDGF-BB treatment was ineffective. However, in surface-irradiated rats, PDGF-BB treatment recruited macrophages into wounds and partially reversed impaired healing on day 7 (p < 0.005) and day 12 (p < 0.001). PDGF-BB-treated wounds were 50 percent stronger than the paired control wounds. The results suggest PDGF requires bone marrow-derived cells, likely wound macrophages, for activity and that it may be useful as a topical agent in postirradiation surgical incisions.
AB - This study examined the potential influence of platelet-derived growth factor-BB homodimers (PDGF-BB) on surgical incisions in irradiated animals with depressed wound healing. Rats were irradiated with either 800 rods total body or 2,500 rads surface irradiation. Parallel dorsal skin incisions were made 2 days later, and PDGF-BB was applied topically a single time to one of two incisions. In total body-irradiated rats, bone marrow-derived elements were severely depressed, wound macrophages were virtually eliminated, and PDGF-BB treatment was ineffective. However, in surface-irradiated rats, PDGF-BB treatment recruited macrophages into wounds and partially reversed impaired healing on day 7 (p < 0.005) and day 12 (p < 0.001). PDGF-BB-treated wounds were 50 percent stronger than the paired control wounds. The results suggest PDGF requires bone marrow-derived cells, likely wound macrophages, for activity and that it may be useful as a topical agent in postirradiation surgical incisions.
UR - http://www.scopus.com/inward/record.url?scp=0024441779&partnerID=8YFLogxK
U2 - 10.1016/0002-9610(89)90131-1
DO - 10.1016/0002-9610(89)90131-1
M3 - Article
C2 - 2508504
AN - SCOPUS:0024441779
SN - 0002-9610
VL - 158
SP - 345
EP - 350
JO - The American Journal of Surgery
JF - The American Journal of Surgery
IS - 4
ER -