Retina-specific cis-elements and binding nuclear proteins of carp rhodopsin gene

Gwo Chin Ma; Tzu Ming Wang; Chih Ying Su; Yi Lei Wang; Shiming Chen; Huai Jen Tsai

doi:10.1016/S0014-5793(01)03058-7

Retina-specific cis-elements and binding nuclear proteins of carp rhodopsin gene

Gwo Chin Ma, Tzu Ming Wang, Chih Ying Su, Yi Lei Wang, Shiming Chen, Huai Jen Tsai

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The upstream cis-elements controlling the retina-specific expression of carp rhodopsin gene were fully characterized in vivo. Transgenic studies demonstrated that both carp neural retina leucine zipper response-like element (cNRE, within nucleotides (nt) -63 to -75) and carp-specific element (CSE, nt -46 to -52) were crucial to reporter gene expression in medaka retinae. The retina-specific expression rates of embryos injected with nt -1 to -641 and longer fragments were much higher than those of embryos injected with nt -1 to -138 and shorter fragments, indicating that an enhancer is located in the nt -138 to -641 region. Retinal extracts and the probe BAT-1 (nt -90 to -120) formed two DNA-protein complexes, B1 and B2. Retinal extracts and the probes cNRE and CSE formed the complexes N1 and C1, respectively. The protein factors in B1 and C1 were mammal-like cone-rod homeobox proteins.

Original language	English
Pages (from-to)	265-271
Number of pages	7
Journal	FEBS Letters
Volume	508
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(01)03058-7
State	Published - Nov 16 2001

Keywords

Carp
Photoreceptor
Regulatory element
Rhodopsin
Transgenic fish

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10.1016/S0014-5793(01)03058-7

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title = "Retina-specific cis-elements and binding nuclear proteins of carp rhodopsin gene",

abstract = "The upstream cis-elements controlling the retina-specific expression of carp rhodopsin gene were fully characterized in vivo. Transgenic studies demonstrated that both carp neural retina leucine zipper response-like element (cNRE, within nucleotides (nt) -63 to -75) and carp-specific element (CSE, nt -46 to -52) were crucial to reporter gene expression in medaka retinae. The retina-specific expression rates of embryos injected with nt -1 to -641 and longer fragments were much higher than those of embryos injected with nt -1 to -138 and shorter fragments, indicating that an enhancer is located in the nt -138 to -641 region. Retinal extracts and the probe BAT-1 (nt -90 to -120) formed two DNA-protein complexes, B1 and B2. Retinal extracts and the probes cNRE and CSE formed the complexes N1 and C1, respectively. The protein factors in B1 and C1 were mammal-like cone-rod homeobox proteins.",

keywords = "Carp, Photoreceptor, Regulatory element, Rhodopsin, Transgenic fish",

author = "Ma, {Gwo Chin} and Wang, {Tzu Ming} and Su, {Chih Ying} and Wang, {Yi Lei} and Shiming Chen and Tsai, {Huai Jen}",

note = "Funding Information: We gratefully acknowledge Anand Swaroop, University of Michigan, for providing us with purified anti-Nrl polyclonal antibodies. We also thank Hung-Kuang Peng, the Tzu-Pei branch of the Taiwan Fisheries Research Institute, for providing carp. S.C. is a recipient of a Career Development Award from Research to Prevent Blindness. This work was supported by a Grant (NSC 88-2311-B002-046) to H.J.T. from the National Science Council, Taiwan.",

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AU - Ma, Gwo Chin

AU - Wang, Tzu Ming

AU - Su, Chih Ying

AU - Wang, Yi Lei

AU - Chen, Shiming

AU - Tsai, Huai Jen

N1 - Funding Information: We gratefully acknowledge Anand Swaroop, University of Michigan, for providing us with purified anti-Nrl polyclonal antibodies. We also thank Hung-Kuang Peng, the Tzu-Pei branch of the Taiwan Fisheries Research Institute, for providing carp. S.C. is a recipient of a Career Development Award from Research to Prevent Blindness. This work was supported by a Grant (NSC 88-2311-B002-046) to H.J.T. from the National Science Council, Taiwan.

PY - 2001/11/16

Y1 - 2001/11/16

N2 - The upstream cis-elements controlling the retina-specific expression of carp rhodopsin gene were fully characterized in vivo. Transgenic studies demonstrated that both carp neural retina leucine zipper response-like element (cNRE, within nucleotides (nt) -63 to -75) and carp-specific element (CSE, nt -46 to -52) were crucial to reporter gene expression in medaka retinae. The retina-specific expression rates of embryos injected with nt -1 to -641 and longer fragments were much higher than those of embryos injected with nt -1 to -138 and shorter fragments, indicating that an enhancer is located in the nt -138 to -641 region. Retinal extracts and the probe BAT-1 (nt -90 to -120) formed two DNA-protein complexes, B1 and B2. Retinal extracts and the probes cNRE and CSE formed the complexes N1 and C1, respectively. The protein factors in B1 and C1 were mammal-like cone-rod homeobox proteins.

AB - The upstream cis-elements controlling the retina-specific expression of carp rhodopsin gene were fully characterized in vivo. Transgenic studies demonstrated that both carp neural retina leucine zipper response-like element (cNRE, within nucleotides (nt) -63 to -75) and carp-specific element (CSE, nt -46 to -52) were crucial to reporter gene expression in medaka retinae. The retina-specific expression rates of embryos injected with nt -1 to -641 and longer fragments were much higher than those of embryos injected with nt -1 to -138 and shorter fragments, indicating that an enhancer is located in the nt -138 to -641 region. Retinal extracts and the probe BAT-1 (nt -90 to -120) formed two DNA-protein complexes, B1 and B2. Retinal extracts and the probes cNRE and CSE formed the complexes N1 and C1, respectively. The protein factors in B1 and C1 were mammal-like cone-rod homeobox proteins.

KW - Carp

KW - Photoreceptor

KW - Regulatory element

KW - Rhodopsin

KW - Transgenic fish

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Retina-specific cis-elements and binding nuclear proteins of carp rhodopsin gene

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Keywords

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