TY - JOUR
T1 - Resynchronization dynamics reveal that the ventral entrains the dorsal suprachiasmatic nucleus
AU - Taylor, Stephanie R.
AU - Wang, Thomas J.
AU - Granados-Fuentes, Daniel
AU - Herzog, Erik D.
N1 - Funding Information:
We thank Scott Pauls for image pre-processing code and John Abel, Matthew Tso, and Paula Nieto for helpful comments. This work was supported by a Clare Boothe Luce Assistant professorship (SRT, funded by the Henry Luce Foundation) and by an NIH grant R01GM096873 from the National Institute of General Medical Sciences (SRT and EDH).
Publisher Copyright:
© 2016 The Author(s).
PY - 2017/2
Y1 - 2017/2
N2 - Although the suprachiasmatic nucleus (SCN) has long been considered the master circadian clock in mammals, the topology of the connections that synchronize daily rhythms among SCN cells is not well understood. We combined experimental and computational methods to infer the directed interactions that mediate circadian synchrony between regions of the SCN. We analyzed PERIOD2 (PER2) expression from SCN slices during and after treatment with tetrodotoxin, allowing us to map connections as cells resynchronized their daily cycling following blockade and restoration of cell-cell communication. Using automated analyses, we found that cells in the dorsal SCN stabilized their periods slower than those in the ventral SCN. A phaseamplitude computational model of the SCN revealed that, to reproduce the experimental results: (1) the ventral SCN had to be more densely connected than the dorsal SCN and (2) the ventral SCN needed strong connections to the dorsal SCN. Taken together, these results provide direct evidence that the ventral SCN entrains the dorsal SCN in constant conditions.
AB - Although the suprachiasmatic nucleus (SCN) has long been considered the master circadian clock in mammals, the topology of the connections that synchronize daily rhythms among SCN cells is not well understood. We combined experimental and computational methods to infer the directed interactions that mediate circadian synchrony between regions of the SCN. We analyzed PERIOD2 (PER2) expression from SCN slices during and after treatment with tetrodotoxin, allowing us to map connections as cells resynchronized their daily cycling following blockade and restoration of cell-cell communication. Using automated analyses, we found that cells in the dorsal SCN stabilized their periods slower than those in the ventral SCN. A phaseamplitude computational model of the SCN revealed that, to reproduce the experimental results: (1) the ventral SCN had to be more densely connected than the dorsal SCN and (2) the ventral SCN needed strong connections to the dorsal SCN. Taken together, these results provide direct evidence that the ventral SCN entrains the dorsal SCN in constant conditions.
KW - Circadian
KW - Computational model
KW - Entrainment
KW - Period gene
KW - SCN
KW - Vasoactive intestinal polypeptide
KW - Vasopressin
UR - http://www.scopus.com/inward/record.url?scp=85018683406&partnerID=8YFLogxK
U2 - 10.1177/0748730416680904
DO - 10.1177/0748730416680904
M3 - Article
C2 - 28326909
AN - SCOPUS:85018683406
SN - 0748-7304
VL - 32
SP - 35
EP - 47
JO - Journal of Biological Rhythms
JF - Journal of Biological Rhythms
IS - 1
ER -