Resurrection of an urbilaterian U1A/U2B″/SNF protein

Sandra G. Williams, Michael J. Harms, Kathleen B. Hall

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The U1A/U2B″/SNF family of proteins found in the U1 and U2 spliceosomal small nuclear ribonucleoproteins is highly conserved. In spite of the high degree of sequence and structural conservation, modern members of this protein family have unique RNA binding properties. These differences have necessarily resulted from evolutionary processes, and therefore, we reconstructed the protein phylogeny in order to understand how and when divergence occurred and how protein function has been modulated. Contrary to the conventional understanding of an ancient human U1A/U2B″ gene duplication, we show that the last common ancestor of bilaterians contained a single ancestral protein (URB). The gene for URB was synthesized, the protein was overexpressed and purified, and we assessed RNA binding to modern snRNA sequences. We find that URB binds human and Drosophila U1 snRNA SLII and U2 snRNA SLIV with higher affinity than do modern homologs, suggesting that both Drosophila SNF and human U1A/U2B″ have evolved into weaker binders of one RNA or both RNAs.

Original languageEnglish
Pages (from-to)3846-3862
Number of pages17
JournalJournal of Molecular Biology
Volume425
Issue number20
DOIs
StatePublished - Oct 23 2013

Keywords

  • RNA binding
  • RRM
  • U1A protein
  • ancestral reconstruction
  • protein phylogeny

Fingerprint

Dive into the research topics of 'Resurrection of an urbilaterian U1A/U2B″/SNF protein'. Together they form a unique fingerprint.

Cite this