Resting-state functional connectivity imaging of the mouse brain using photoacoustic tomography

Mohammadreza Nasiriavanaki, Jun Xia, Hanlin Wan, Adam Q. Bauer, Joseph P. Culver, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

16 Scopus citations


Resting-state functional connectivity (RSFC) imaging is an emerging neuroimaging approach that aims to identify spontaneous cerebral hemodynamic fluctuations and their associated functional connections. Clinical studies have demonstrated that RSFC is altered in brain disorders such as stroke, Alzheimer's, autism, and epilepsy. However, conventional neuroimaging modalities cannot easily be applied to mice, the most widely used model species for human brain disease studies. For instance, functional magnetic resonance imaging (fMRI) of mice requires a very high magnetic field to obtain a sufficient signal-to-noise ratio and spatial resolution. Functional connectivity mapping with optical intrinsic signal imaging (fcOIS) is an alternative method. Due to the diffusion of light in tissue, the spatial resolution of fcOIS is limited, and experiments have been performed using an exposed skull preparation. In this study, we show for the first time, the use of photoacoustic computed tomography (PACT) to noninvasively image resting-state functional connectivity in the mouse brain, with a large field of view and a high spatial resolution. Bilateral correlations were observed in eight regions, as well as several subregions. These findings agreed well with the Paxinos mouse brain atlas. This study showed that PACT is a promising, non-invasive modality for small-animal functional brain imaging.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2014
ISBN (Print)9780819498564
StatePublished - Mar 3 2014
EventPhotons Plus Ultrasound: Imaging and Sensing 2014 - San Francisco, CA, United States
Duration: Feb 2 2014Feb 5 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2014
Country/TerritoryUnited States
CitySan Francisco, CA


  • Electrical Stimulation
  • Functional Imaging
  • Photoacoustic Tomography


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