TY - JOUR
T1 - Response of Xanthomonas citri pv. malvacearum Isolates to Cotton Differing in Susceptibility to the Bacterium and Their Predicted Type III Effectors
AU - Wheeler, Terry A.
AU - Harris, Taylor
AU - Bart, Rebecca S.
AU - Isakeit, Thomas
AU - Woodward, Jason
AU - Allen, Tom W.
AU - Kemerait, Robert C.
N1 - Funding Information:
We thank CIRAD (Cotton germplasm collection managed by the French Centre de Coop´eration international en recherche agronomique pour le d´eveloppement, located in Montpellier, France) for sending seed of S295. Funding: This work was funded by NIFA IPM project TEX09672 and NSF award 1928344 to RSB.
Funding Information:
Funding: This work was funded by NIFA IPM project TEX09672 and NSF award 1928344 to RSB.
Publisher Copyright:
© 2022
PY - 2022
Y1 - 2022
N2 - From 2015 to 2020, 342 isolates of Xanthomonas citri pv. malvacearum (Xcm) were obtained from cotton fields in Texas, including 64 isolates collected from symptomatic cultivars that were thought to be resistant to race 18 strains of Xcm (the predominant race in the United States). Symptoms on highly resistant cultivars prompted concern that a new race of Xcm was present. The 342 isolates were inoculated on a race 18 susceptible (DP 1747NR B2XF) and race 18 resistant (S295) cotton cultivar, and none of the isolates caused blight-type symptoms (water soaking and chlorosis) on S295, indicating that the B12 resistant gene was still beneficial for disease management. Four cultivars, varying in their field response to bacterial blight, were inoculated with each of 17 isolates of Xcm, and the incidence of plants exhibiting bacterial blight symptoms averaged 87% for DP 1747NR B2XF, 51% for partially susceptible NG 4936 B3XF, 16% for partially resistant DP 1646 B2XF, and 0% for S295. Xcm isolates from Texas (11), Georgia (1), and Oklahoma (1) were sequenced, and their type three effectors (T3Es) were predicted. All isolates (Georgia, Oklahoma, and Texas) had the same T3E proteins as previously identified Xcm race 18 isolates (tested for 25 genes), including XopJ. Race 1, 2, 3, and 12 of Xcm included in the comparisons were all missing the XopJ gene. Use of cultivars with the B12 gene is an effective strategy to manage bacterial blight of cotton.
AB - From 2015 to 2020, 342 isolates of Xanthomonas citri pv. malvacearum (Xcm) were obtained from cotton fields in Texas, including 64 isolates collected from symptomatic cultivars that were thought to be resistant to race 18 strains of Xcm (the predominant race in the United States). Symptoms on highly resistant cultivars prompted concern that a new race of Xcm was present. The 342 isolates were inoculated on a race 18 susceptible (DP 1747NR B2XF) and race 18 resistant (S295) cotton cultivar, and none of the isolates caused blight-type symptoms (water soaking and chlorosis) on S295, indicating that the B12 resistant gene was still beneficial for disease management. Four cultivars, varying in their field response to bacterial blight, were inoculated with each of 17 isolates of Xcm, and the incidence of plants exhibiting bacterial blight symptoms averaged 87% for DP 1747NR B2XF, 51% for partially susceptible NG 4936 B3XF, 16% for partially resistant DP 1646 B2XF, and 0% for S295. Xcm isolates from Texas (11), Georgia (1), and Oklahoma (1) were sequenced, and their type three effectors (T3Es) were predicted. All isolates (Georgia, Oklahoma, and Texas) had the same T3E proteins as previously identified Xcm race 18 isolates (tested for 25 genes), including XopJ. Race 1, 2, 3, and 12 of Xcm included in the comparisons were all missing the XopJ gene. Use of cultivars with the B12 gene is an effective strategy to manage bacterial blight of cotton.
KW - Bacterial blight
KW - cotton
KW - race
KW - resistance
KW - type III effectors
UR - http://www.scopus.com/inward/record.url?scp=85125037983&partnerID=8YFLogxK
U2 - 10.1094/PHP-06-21-0090-RS
DO - 10.1094/PHP-06-21-0090-RS
M3 - Article
AN - SCOPUS:85125037983
SN - 1535-1025
VL - 23
SP - 40
EP - 44
JO - Plant Health Progress
JF - Plant Health Progress
IS - 1
ER -