Respiratory rate and atp content of stria vascularis of guinea pig in vitro

Daniel C. Marcus, Ruediger Thalmann, Nancy Y. Marcus

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Abstract

Stria vascularis from guinea pig cochleae was incubated in vitro to determine its metabolic response to variations in substrate arid ion composition of the incubation medium. The respiratory rate at 37° C in a medium containing glucose and pyruvate as substrate was 17.3 ± 1.33 (SEM, n = 51) µ1 O2/mg dry weight-hour. The stria could not maintain constant respiration by relying solely upon endogenous fuel stores. With substrate supplied, the ATP level could be maintained at about 73% of that existing in vivo. Glucose appears to bean adequate substrate for stria in vitro since glutamate, pyruvate, and fuma-rate did not increase the respiratory rate. Succinate increased respiration markedly but did not increase the ATP level. Ouabain (10-4 M) caused a 48% decrease in the respiratory rate. Incubation in NaMree and K+-free medium, each resulted in irreversible decrease of respiratory rate comparable to (or greater than) that caused by ouabain. These data are in accord with the high activity of Na+-K+-ATPase in the stria and the pronounced sensitivity of the endolymphatic potential to ouabain.

Original languageEnglish
Pages (from-to)1825-1835
Number of pages11
JournalLaryngoscope
Volume88
Issue number11
StatePublished - Nov 1978

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    Marcus, D. C., Thalmann, R., & Marcus, N. Y. (1978). Respiratory rate and atp content of stria vascularis of guinea pig in vitro. Laryngoscope, 88(11), 1825-1835.