Resonance energy transfer as an emerging technique for monitoring protein-protein interactions in vivo: BRET vs. FRET

Yao Xu, Akihito Kanauchi, David W. Piston, Carl Hirschie Johnson

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

5 Scopus citations

Abstract

Protein-protein interactions are known to play an important role in a variety of biochemical systems. To date, thousands of protein-protein interactions have been identified by using the conventional two-hybrid system, but this method is limited in that the interaction must occur in the yeast nucleus. This means interactions that strictly depend upon cell-type-specific processing or compartmentalization will not be detected. Therefore, a number of new methods have been developed recently that rely on reconstitution of biochemical function in vivo, such as fluorescence resonance energy transfer (FRET), protein mass spectrometry, or evanescent wave.

Original languageEnglish
Title of host publicationLuminescence Biotechnology
Subtitle of host publicationInstruments and Applications
PublisherCRC Press
Pages529-538
Number of pages10
ISBN (Electronic)9781420041804
ISBN (Print)9780849307195
DOIs
StatePublished - Jan 1 2001

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