Abstract
Protein-protein interactions are known to play an important role in a variety of biochemical systems. To date, thousands of protein-protein interactions have been identified by using the conventional two-hybrid system, but this method is limited in that the interaction must occur in the yeast nucleus. This means interactions that strictly depend upon cell-type-specific processing or compartmentalization will not be detected. Therefore, a number of new methods have been developed recently that rely on reconstitution of biochemical function in vivo, such as fluorescence resonance energy transfer (FRET), protein mass spectrometry, or evanescent wave.
Original language | English |
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Title of host publication | Luminescence Biotechnology |
Subtitle of host publication | Instruments and Applications |
Publisher | CRC Press |
Pages | 529-538 |
Number of pages | 10 |
ISBN (Electronic) | 9781420041804 |
ISBN (Print) | 9780849307195 |
DOIs | |
State | Published - Jan 1 2001 |