Protein-protein interactions are known to play an important role in a variety of biochemical systems. To date, thousands of protein-protein interactions have been identified by using the conventional two-hybrid system, but this method is limited in that the interaction must occur in the yeast nucleus. This means interactions that strictly depend upon cell-type-specific processing or compartmentalization will not be detected. Therefore, a number of new methods have been developed recently that rely on reconstitution of biochemical function in vivo, such as fluorescence resonance energy transfer (FRET), protein mass spectrometry, or evanescent wave.
|Title of host publication||Luminescence Biotechnology|
|Subtitle of host publication||Instruments and Applications|
|Number of pages||10|
|State||Published - Jan 1 2001|