TY - JOUR
T1 - Resection-induced intestinal adaptation and the role of enteric smooth muscle
AU - Martin, Colin A.
AU - Bernabe, Kathryn Q.
AU - Taylor, Janice A.
AU - Nair, Rajalakshmi
AU - Paul, Richard J.
AU - Guo, Jun
AU - Erwin, Christopher R.
AU - Warner, Brad W.
N1 - Funding Information:
Supported by National Institutes of Health: RO1 DK53234, (BWW), and Digestive Disease Research Development Core Center of Cincinnati—R24 DK064403.
PY - 2008/6
Y1 - 2008/6
N2 - Background: Intestinal adaptation after massive small bowel resection (SBR) involves all layers of the bowel wall. Most prior work has focused on changes that occur in the intestinal mucosa. However, the contribution of the underlying intestinal smooth muscle (ISM) to the overall adaptation response remains unclear. Methods: Male C57BL/6 or waved-2 (diminished activity of the epidermal growth factor receptor) mice underwent a 50% proximal SBR or sham operation, and the remnant ileum was harvested 3, 7, and 28 days. Markers of adaptation (villus height, bowel length, circumference, and ISM thickness) and ISM proliferation were recorded. Contractility was measured by attaching the distal ileum to strain gauge transducers and exposed to varying doses of carbachol. Results: Intestinal smooth muscle thickness was unchanged at any given time-point after resection; however, the bowel caliber and length were increased, and augmented rates of ISM proliferation were identified. Contractility was increased at 7 days after SBR. Waved-2 mice demonstrated minimal proliferation or intestinal lengthening in response to SBR. Conclusion: Compared with resection-induced thickening of the mucosa, proliferative changes in the ISM are unique and primarily affect bowel caliber, length, and contractility. Epidermal growth factor receptor signaling appears to play a significant role in adaptation of the ISM cellular compartment.
AB - Background: Intestinal adaptation after massive small bowel resection (SBR) involves all layers of the bowel wall. Most prior work has focused on changes that occur in the intestinal mucosa. However, the contribution of the underlying intestinal smooth muscle (ISM) to the overall adaptation response remains unclear. Methods: Male C57BL/6 or waved-2 (diminished activity of the epidermal growth factor receptor) mice underwent a 50% proximal SBR or sham operation, and the remnant ileum was harvested 3, 7, and 28 days. Markers of adaptation (villus height, bowel length, circumference, and ISM thickness) and ISM proliferation were recorded. Contractility was measured by attaching the distal ileum to strain gauge transducers and exposed to varying doses of carbachol. Results: Intestinal smooth muscle thickness was unchanged at any given time-point after resection; however, the bowel caliber and length were increased, and augmented rates of ISM proliferation were identified. Contractility was increased at 7 days after SBR. Waved-2 mice demonstrated minimal proliferation or intestinal lengthening in response to SBR. Conclusion: Compared with resection-induced thickening of the mucosa, proliferative changes in the ISM are unique and primarily affect bowel caliber, length, and contractility. Epidermal growth factor receptor signaling appears to play a significant role in adaptation of the ISM cellular compartment.
KW - Adaptation
KW - Enteric smooth muscle
KW - Epidermal growth factor receptor
KW - Hyperplasia
KW - Intestinal contractility
KW - Intestinal resection
UR - http://www.scopus.com/inward/record.url?scp=44949199334&partnerID=8YFLogxK
U2 - 10.1016/j.jpedsurg.2008.02.015
DO - 10.1016/j.jpedsurg.2008.02.015
M3 - Article
C2 - 18558175
AN - SCOPUS:44949199334
SN - 0022-3468
VL - 43
SP - 1011
EP - 1017
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
IS - 6
ER -