Requirement of Rho-family GTPases in the invasion of type 1-piliated uropathogenic Escherichia coli

Juan J. Martinez, Scott J. Hultgren

Research output: Contribution to journalArticle

100 Scopus citations


Bladder infections caused by uropathogenic Escherichia coli (UPEC) depends on the ability of E. coli to express type 1 pili. The adhesive component of the pilus, FimH, mediates the invasion of E. coli into the bladder epithelium, a mechanism that facilitates the survival and persistence of E. coli in the bladder. The invasion mechanism requires actin polymerization, focal adhesion kinase phosphorylation and PI 3-kinase activation as well as the formation of FAK/PI 3-kinase and downstream vinculin/α-actinin complexes. In this study, we report a role for Rho-GTPase family members, namely RhoA, Cdc42 and Rac1, in the invasion process. Internalization of type 1-piliated E. coli (fimH+) and FimH-coated microspheres was inhibited by compactin, a pan-Rho-GTPase inhibitor and dominant negative isoforms of Rac1 and Cdc42. Expression of active Rac1 induced an internalization of E. coli that was insensitive to wortmannin and genistein. Expression of constitutively active Cdc42 induced the formation of FAK/PI 3-kinase and vinculin/α-actinin complexes whereas active Rac1 induced only a vinculin/α-actinin complex. Taken together, these data suggest that FimH-mediated invasion is dependent on GTP-binding protein activity that involves Cdc42 and PI 3-kinase activation probably upstream of Rac1.

Original languageEnglish
Pages (from-to)19-28
Number of pages10
JournalCellular microbiology
Issue number1
StatePublished - Feb 13 2002

Fingerprint Dive into the research topics of 'Requirement of Rho-family GTPases in the invasion of type 1-piliated uropathogenic Escherichia coli'. Together they form a unique fingerprint.

  • Cite this