Requirement of proliferating cell nuclear antigen in RAD6-dependent postreplicational DNA repair

Carlos A. Torres-Ramos, Bonita L. Yoder, Peter M.J. Burgers, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

The proliferating cell nuclear antigen (PCNA) acts as a processivity factor for replicative DNA polymerases and is essential for DNA replication. In vitro studies have suggested a role for PCNA in the repair synthesis step of nucleotide excision repair, and PCNA interacts with the cyclin-dependent kinase inhibitor p21. However, because of the lack of genetic evidence, it is not clear which of the DNA repair processes are in tact affected by PCNA in vivo. Here, we describe a PCNA mutation, pol30-46, that confers ultraviolet (UV) sensitivity but has no effect on growth or cell cycle progression, and the mutant pcna interacts normally with DNA polymerase δ and ε. Genetic studies indicate that the pol30-46 mutation is specifically defective in RAD6-dependent postreplicational repair of UV damaged DNA, and this mutation impairs the error-free mode of bypass repair. These results implicate a role for PCNA as an intermediary between DNA replication and postreplicational DNA repair.

Original languageEnglish
Pages (from-to)9676-9681
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number18
DOIs
StatePublished - Sep 3 1996

Keywords

  • REV3
  • Saccharomyces cerevisiae
  • nonmutagenic and mutagenic bypass

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