Requirement for the SLP-76 adaptor GADS in T cell development

J. Yoder; C. Pham; Y. M. Iizuka; O. Kanagawa; S. K. Liu; J. McGlade; A. M. Cheng

doi:10.1126/science.1057176

Requirement for the SLP-76 adaptor GADS in T cell development

J. Yoder, C. Pham, Y. M. Iizuka, O. Kanagawa, S. K. Liu, J. McGlade, A. M. Cheng

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124 Scopus citations

Abstract

GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADS^- CD4^-CD8^- thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS^- thymocytes failed to respond to CD3 cross-linking in vivo and were impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS^- thymocytes. These observations indicate that GADS is a critical adaptor for CD3 signaling.

Original language	English
Pages (from-to)	1987-1991
Number of pages	5
Journal	Science
Volume	291
Issue number	5510
DOIs	https://doi.org/10.1126/science.1057176
State	Published - Mar 9 2001

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abstract = "GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADS- CD4-CD8- thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS- thymocytes failed to respond to CD3 cross-linking in vivo and were impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS- thymocytes. These observations indicate that GADS is a critical adaptor for CD3 signaling.",

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AU - Pham, C.

AU - Iizuka, Y. M.

AU - Kanagawa, O.

AU - Liu, S. K.

AU - McGlade, J.

AU - Cheng, A. M.

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N2 - GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADS- CD4-CD8- thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS- thymocytes failed to respond to CD3 cross-linking in vivo and were impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS- thymocytes. These observations indicate that GADS is a critical adaptor for CD3 signaling.

AB - GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADS- CD4-CD8- thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS- thymocytes failed to respond to CD3 cross-linking in vivo and were impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS- thymocytes. These observations indicate that GADS is a critical adaptor for CD3 signaling.

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Requirement for the SLP-76 adaptor GADS in T cell development

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