Reproducibility of creatine kinase reaction kinetics in human heart: A 31P time-dependent saturation transfer spectroscopy study

Adil Bashir, Robert Gropler

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Creatine kinase (CK) is essential for the buffering and rapid regeneration of adenosine triphosphate (ATP) in heart tissue. Herein, we demonstrate a 31P MRS protocol to quantify CK reaction kinetics in human myocardium at 3 T. Furthermore, we sought to quantify the test-retest reliability of the measured metabolic parameters. The method localizes the 31P signal from the heart using modified one-dimensional image-selected in vivo spectroscopy (ISIS), and a time-dependent saturation transfer (TDST) approach was used to measure CK reaction parameters. Fifteen healthy volunteers (22 measurements in total) were tested. The CK reaction rate constant (kf) was 0.32±0.05 s-1 and the coefficient of variation (CV) was 15.62%. The intrinsic T1 for phosphocreatine (PCr) was 7.36±1.79 s with CV=24.32%. These values are consistent with those reported previously. The PCr/ATP ratio was equal to 1.94±0.15 with CV=7.73%, which is within the range of healthy subjects. The reproducibility of the technique was tested in seven subjects and inferred parameters, such as kf and T1, exhibited good reliability [intraclass correlation coefficient (ICC) of 0.90 and 0.79 for kf and T1, respectively). The reproducibility data provided in this study will enable the calculation of the power and sample sizes required for clinical and research studies. The technique will allow for the examination of cardiac energy metabolism in clinical and research studies, providing insight into the relationship between energy deficit and functional deficiency in the heart.

Original languageEnglish
Pages (from-to)663-671
Number of pages9
JournalNMR in biomedicine
Volume27
Issue number6
DOIs
StatePublished - Jun 2014

Keywords

  • Adenosine triphosphate (ATP)
  • Creatine kinase
  • Heart
  • Image-selected in vivo spectroscopy (ISIS)
  • Reproducibility
  • Saturation transfer
  • Time-dependent saturation transfer (TDST)

Fingerprint

Dive into the research topics of 'Reproducibility of creatine kinase reaction kinetics in human heart: A 31P time-dependent saturation transfer spectroscopy study'. Together they form a unique fingerprint.

Cite this