TY - JOUR
T1 - Repopulation of the irradiation damaged lung with bone marrow-derived cells
AU - Bernard, Mark E.
AU - Kim, Hyun
AU - Rajagopalan, Malolan S.
AU - Stone, Brandon
AU - Salimi, Umar
AU - Rwigema, Jean Claude
AU - Epperly, Michael W.
AU - Shen, Hongmei
AU - Goff, Julie P.
AU - Franicola, Darcy
AU - Dixon, Tracy
AU - Cao, Shaonan
AU - Zhang, Xichen
AU - Wang, Hong
AU - Stolz, Donna B.
AU - Greenberger, Joel S.
PY - 2012
Y1 - 2012
N2 - Aim: The effect of lung irradiation on reduction of lung stem cells and repopulation with bone marrow-derived cells was measured. Materials and Methods: Expression of green fluorescent protein positive cells (GFP+) in the lungs of thoracic irradiated FVB/NHsd mice (Harlan Sprague Dawley, Indianapolis, IN, USA) was determined. This was compared to the repopulation of bone marrow-derived cells found in the lungs from naphthalene treated male FVBINHsd mice and gangciclovir (GCV) treated FeVBN GFP+ male marrow chimeric HSV-TK-CCSP. The level of mRNA for lung stem cell markers clara cell (CCSP), epithelium 1 (FOXJ1) and surfactant protein C (SP-C), and sorted single cells positive for marrow origin epithelial cells (GFP+CD45 -) was measured. Results: The expression of pulmonary stem cells as determined by PCR was reduced most by GCV, then naphthalene, and least by thoracic irradiation. Irradiation, like GCV, reduced mRNA expression of CCSP, CYP2F2, and FOXJ1, while naphthalene reduced that of CCSP and CYP2F2. Ultrastructural analysis showed GFP+ pulmonary cells of bone marrow origin, with the highest frequency being found in GCV-treated groups. Conclusion: Bone marrow progenitor cells may not participate in the repopulation of the lung following irradiation.
AB - Aim: The effect of lung irradiation on reduction of lung stem cells and repopulation with bone marrow-derived cells was measured. Materials and Methods: Expression of green fluorescent protein positive cells (GFP+) in the lungs of thoracic irradiated FVB/NHsd mice (Harlan Sprague Dawley, Indianapolis, IN, USA) was determined. This was compared to the repopulation of bone marrow-derived cells found in the lungs from naphthalene treated male FVBINHsd mice and gangciclovir (GCV) treated FeVBN GFP+ male marrow chimeric HSV-TK-CCSP. The level of mRNA for lung stem cell markers clara cell (CCSP), epithelium 1 (FOXJ1) and surfactant protein C (SP-C), and sorted single cells positive for marrow origin epithelial cells (GFP+CD45 -) was measured. Results: The expression of pulmonary stem cells as determined by PCR was reduced most by GCV, then naphthalene, and least by thoracic irradiation. Irradiation, like GCV, reduced mRNA expression of CCSP, CYP2F2, and FOXJ1, while naphthalene reduced that of CCSP and CYP2F2. Ultrastructural analysis showed GFP+ pulmonary cells of bone marrow origin, with the highest frequency being found in GCV-treated groups. Conclusion: Bone marrow progenitor cells may not participate in the repopulation of the lung following irradiation.
KW - Bone marrow
KW - CCSP
KW - FOXJ1
KW - GCV
KW - GFP
KW - Lung repair
KW - Surfactant protein C
UR - http://www.scopus.com/inward/record.url?scp=84855367953&partnerID=8YFLogxK
M3 - Article
C2 - 22210711
AN - SCOPUS:84855367953
SN - 0258-851X
VL - 26
SP - 9
EP - 18
JO - In Vivo
JF - In Vivo
IS - 1
ER -