TY - JOUR
T1 - Replication of Norovirus in cell culture reveals a tropism for dendritic cells and macrophages
AU - Wobus, Christiane E.
AU - Karst, Stephanie M.
AU - Thackray, Larissa B.
AU - Chang, Kyeong Ok
AU - Sosnovtsev, Stanislav V.
AU - Belliot, Gaël
AU - Krug, Anne
AU - Mackenzie, Jason M.
AU - Green, Kim Y.
AU - Virgin IV, Herbert W.
PY - 2004/12
Y1 - 2004/12
N2 - Noroviruses are understudied because these important enteric pathogens have not been cultured to date. We found that the norovirus murine norovirus 1 (MNV-1) infects macrophage-like cells in vivo and replicates in cultured primary dendritic cells and macrophages. MNV-1 growth was inhibited by the interferon-αβ receptor and STAT-1, and was associated with extensive rearrangements of intracellular membranes. An amino acid substitution in the capsid protein of serially passaged MNV-1 was associated with virulence attenuation in vivo. This is the first report of replication of a norovirus in cell culture. The capacity of MNV-1 to replicate in a STAT-1-regulated fashion and the unexpected tropism of a norovirus for cells of the hematopoietic lineage provide important insights into norovirus biology. Copyright:
AB - Noroviruses are understudied because these important enteric pathogens have not been cultured to date. We found that the norovirus murine norovirus 1 (MNV-1) infects macrophage-like cells in vivo and replicates in cultured primary dendritic cells and macrophages. MNV-1 growth was inhibited by the interferon-αβ receptor and STAT-1, and was associated with extensive rearrangements of intracellular membranes. An amino acid substitution in the capsid protein of serially passaged MNV-1 was associated with virulence attenuation in vivo. This is the first report of replication of a norovirus in cell culture. The capacity of MNV-1 to replicate in a STAT-1-regulated fashion and the unexpected tropism of a norovirus for cells of the hematopoietic lineage provide important insights into norovirus biology. Copyright:
UR - http://www.scopus.com/inward/record.url?scp=20044387761&partnerID=8YFLogxK
U2 - 10.1371/journal.pbio.0020432
DO - 10.1371/journal.pbio.0020432
M3 - Article
C2 - 15562321
AN - SCOPUS:20044387761
SN - 1544-9173
VL - 2
JO - PLoS biology
JF - PLoS biology
IS - 12
ER -