Replication of Norovirus in cell culture reveals a tropism for dendritic cells and macrophages

Christiane E. Wobus, Stephanie M. Karst, Larissa B. Thackray, Kyeong Ok Chang, Stanislav V. Sosnovtsev, Gaël Belliot, Anne Krug, Jason M. Mackenzie, Kim Y. Green, Herbert W. Virgin IV

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706 Scopus citations

Abstract

Noroviruses are understudied because these important enteric pathogens have not been cultured to date. We found that the norovirus murine norovirus 1 (MNV-1) infects macrophage-like cells in vivo and replicates in cultured primary dendritic cells and macrophages. MNV-1 growth was inhibited by the interferon-αβ receptor and STAT-1, and was associated with extensive rearrangements of intracellular membranes. An amino acid substitution in the capsid protein of serially passaged MNV-1 was associated with virulence attenuation in vivo. This is the first report of replication of a norovirus in cell culture. The capacity of MNV-1 to replicate in a STAT-1-regulated fashion and the unexpected tropism of a norovirus for cells of the hematopoietic lineage provide important insights into norovirus biology. Copyright:

Original languageEnglish
JournalPLoS biology
Volume2
Issue number12
DOIs
StatePublished - Dec 2004

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