@article{a263443249da4202b7c8230ad3010f57,
title = "Removal of aquaporin-4 from glial and ependymal membranes causes brain water accumulation",
abstract = "There is a constitutive production of water in brain. The efflux routes of this excess water remain to be identified. We used basal brain water content as a proxy for the capacity of water exit routes. Basal brain water content was increased in mice with a complete loss of aquaporin-4 (AQP4) water channels (global Aqp4−/− mice), but not in mice with a selective removal of perivascular AQP4 or in a novel mouse line with a selective deletion of ependymal AQP4 (Foxj1-Cre:Aqp4flox/flox mice). Unique for the global Aqp4−/− mice is the loss of the AQP4 pool subjacent to the pial membrane. Our data suggest that water accumulates in brain when subpial AQP4 is missing, pointing to a critical role of this pool of water channels in brain water exit.",
keywords = "AQP4, Alpha-syntrophin, Aquaporin, Astrocytes, Brain edema, CSF, Cerebrospinal fluid, Endfeet, Ependyma, Extracellular space, Foxj1, Glia, Glymphatic, Interstitial fluid, Neuron-glial, Paravascular, Water homeostasis",
author = "Vindedal, {Gry Fluge} and Thoren, {Anna E.} and Vidar Jensen and Arne Klungland and Yong Zhang and Holtzman, {Michael J.} and Ottersen, {Ole Petter} and Nagelhus, {Erlend A.}",
note = "Funding Information: We thank Mr. Gaute Nesse, Institute of Medical Microbiology, Oslo University Hospital, Rikshospitalet, Oslo, for performing the genotyping and Mrs. Carina V. S. Knudsen for technical assistance. This work was supported by the Research Council of Norway (grants no. 226696 and 240476 ), the European Union's Seventh Framework Programme for research, technological development and demonstration under grant agreement no. 601055 , the National Institutes of Health (NIH) grants HL121791 and HL120153 , and the Letten Foundation . The sponsors had no involvement in study design, data collection, analysis and interpretation of data, and writing the manuscript. Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
month = dec,
day = "1",
doi = "10.1016/j.mcn.2016.10.004",
language = "English",
volume = "77",
pages = "47--52",
journal = "Molecular and Cellular Neuroscience",
issn = "1044-7431",
}