Removal of aquaporin-4 from glial and ependymal membranes causes brain water accumulation

Gry Fluge Vindedal, Anna E. Thoren, Vidar Jensen, Arne Klungland, Yong Zhang, Michael J. Holtzman, Ole Petter Ottersen, Erlend A. Nagelhus

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


There is a constitutive production of water in brain. The efflux routes of this excess water remain to be identified. We used basal brain water content as a proxy for the capacity of water exit routes. Basal brain water content was increased in mice with a complete loss of aquaporin-4 (AQP4) water channels (global Aqp4−/− mice), but not in mice with a selective removal of perivascular AQP4 or in a novel mouse line with a selective deletion of ependymal AQP4 (Foxj1-Cre:Aqp4flox/flox mice). Unique for the global Aqp4−/− mice is the loss of the AQP4 pool subjacent to the pial membrane. Our data suggest that water accumulates in brain when subpial AQP4 is missing, pointing to a critical role of this pool of water channels in brain water exit.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalMolecular and Cellular Neuroscience
StatePublished - Dec 1 2016


  • AQP4
  • Alpha-syntrophin
  • Aquaporin
  • Astrocytes
  • Brain edema
  • CSF
  • Cerebrospinal fluid
  • Endfeet
  • Ependyma
  • Extracellular space
  • Foxj1
  • Glia
  • Glymphatic
  • Interstitial fluid
  • Neuron-glial
  • Paravascular
  • Water homeostasis


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