Regulatory elements of microfibril-associated glycoprotein-1 gene expression in muscle cells

Fernando Segade, Robert P. Mecham

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The Mfap2 gene encodes the microfibril-associated glycoprotein-1 (MAGP1), an extracellular matrix protein of microfibrillar structures. The gene is transcribed from a major transcription start site embedded in a CpG island. Mapping of transcriptionally active regions in the 5′ flanking sequence identified a region, located between nucleotides -339 and -109 as the Mfap2 basal promoter. Site-directed and random mutagenesis demonstrated that a KLF sequence motif at -256/-270, an E-box at -222/-229, and a GC-box at -117/-125, are critical for the promoter function. Using electrophoresis mobility shift assays, we find that the KLF motif mediates the binding of GKLF/KLF4, whereas the E-box is a target for both Upstream Stimulatory Factors 1 and 2, and the GC box at -117/-125 forms complexes with Sp1 and Sp3, but not with Sp4 or AP2α. A sequence element spanning position -150 may represent the binding motif of an uncharacterized transcription factor. The basal transcriptional regulation of Mfap2 in muscle cells is discussed.

Original languageEnglish
Pages (from-to)215-224
Number of pages10
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1731
Issue number3
DOIs
StatePublished - Dec 20 2005

Keywords

  • C2C12
  • KLF4
  • MAGP1
  • Microfibril
  • Myoblast
  • Sp1
  • USF

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