Regulation of the nitric oxide pathway genes by tetrahydrofurandiols: Microarray analysis of MCF-7 human breast cancer cells

Kevin Shoulars; Mary Ann Rodriguez; Trellis Thompson; John Turk; Jan Crowley; Barry M. Markaverich

doi:10.1016/j.canlet.2008.01.048

Regulation of the nitric oxide pathway genes by tetrahydrofurandiols: Microarray analysis of MCF-7 human breast cancer cells

Kevin Shoulars, Mary Ann Rodriguez, Trellis Thompson, John Turk, Jan Crowley, Barry M. Markaverich

Division of Endocrinology, Metabolism & Lipid Research

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

THF-diols (9,12-oxy-10,13-dihydroxyoctadecanoic and 10,13-oxy-9,12-dihydroxyoctadecanoic acids) are endocrine disrupters in rats and mitogens in breast cancer cells. Microarray analyses and real-time PCR analyses on RNA from THF-treated MCF-7 cells revealed a number of genes (caveolin 1, heat shock protein 90α and 90β, vascular endothelial growth factor, ATPase, Ca⁺⁺ transporting, ubiquitous) in the nitric oxide pathway (NOP) were targets for THF-diols. Chromatin immunoprecipitation studies suggest THF-diols modify of histone H4 acetylation at the caveolin 1 promoter via an epigenetic mechanism. These findings are consistent with the well-known involvement of NOP genes in cell proliferation and sexual behavior.

Original language	English
Pages (from-to)	265-273
Number of pages	9
Journal	Cancer Letters
Volume	264
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2008.01.048
State	Published - Jun 18 2008

Keywords

Endocrine disruptor
Growth regulation
Human breast cancer
Microarray
Nitric oxide
Reproduction
Tetrahydrofurandiol

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10.1016/j.canlet.2008.01.048

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@article{003cfecda8314ac186aa1d8e55ad1a42,

title = "Regulation of the nitric oxide pathway genes by tetrahydrofurandiols: Microarray analysis of MCF-7 human breast cancer cells",

abstract = "THF-diols (9,12-oxy-10,13-dihydroxyoctadecanoic and 10,13-oxy-9,12-dihydroxyoctadecanoic acids) are endocrine disrupters in rats and mitogens in breast cancer cells. Microarray analyses and real-time PCR analyses on RNA from THF-treated MCF-7 cells revealed a number of genes (caveolin 1, heat shock protein 90α and 90β, vascular endothelial growth factor, ATPase, Ca++ transporting, ubiquitous) in the nitric oxide pathway (NOP) were targets for THF-diols. Chromatin immunoprecipitation studies suggest THF-diols modify of histone H4 acetylation at the caveolin 1 promoter via an epigenetic mechanism. These findings are consistent with the well-known involvement of NOP genes in cell proliferation and sexual behavior.",

keywords = "Endocrine disruptor, Growth regulation, Human breast cancer, Microarray, Nitric oxide, Reproduction, Tetrahydrofurandiol",

author = "Kevin Shoulars and Rodriguez, \{Mary Ann\} and Trellis Thompson and John Turk and Jan Crowley and Markaverich, \{Barry M.\}",

note = "Funding Information: This research is supported by grants from the National Institute for Environmental Health Sciences (ES-09964), the Office of Research on Women{\textquoteright}s Health and National Cancer Institute (CA 35480).",

year = "2008",

month = jun,

day = "18",

doi = "10.1016/j.canlet.2008.01.048",

language = "English",

volume = "264",

pages = "265--273",

journal = "Cancer Letters",

issn = "0304-3835",

number = "2",

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TY - JOUR

T1 - Regulation of the nitric oxide pathway genes by tetrahydrofurandiols

T2 - Microarray analysis of MCF-7 human breast cancer cells

AU - Shoulars, Kevin

AU - Rodriguez, Mary Ann

AU - Thompson, Trellis

AU - Turk, John

AU - Crowley, Jan

AU - Markaverich, Barry M.

N1 - Funding Information: This research is supported by grants from the National Institute for Environmental Health Sciences (ES-09964), the Office of Research on Women’s Health and National Cancer Institute (CA 35480).

PY - 2008/6/18

Y1 - 2008/6/18

N2 - THF-diols (9,12-oxy-10,13-dihydroxyoctadecanoic and 10,13-oxy-9,12-dihydroxyoctadecanoic acids) are endocrine disrupters in rats and mitogens in breast cancer cells. Microarray analyses and real-time PCR analyses on RNA from THF-treated MCF-7 cells revealed a number of genes (caveolin 1, heat shock protein 90α and 90β, vascular endothelial growth factor, ATPase, Ca++ transporting, ubiquitous) in the nitric oxide pathway (NOP) were targets for THF-diols. Chromatin immunoprecipitation studies suggest THF-diols modify of histone H4 acetylation at the caveolin 1 promoter via an epigenetic mechanism. These findings are consistent with the well-known involvement of NOP genes in cell proliferation and sexual behavior.

AB - THF-diols (9,12-oxy-10,13-dihydroxyoctadecanoic and 10,13-oxy-9,12-dihydroxyoctadecanoic acids) are endocrine disrupters in rats and mitogens in breast cancer cells. Microarray analyses and real-time PCR analyses on RNA from THF-treated MCF-7 cells revealed a number of genes (caveolin 1, heat shock protein 90α and 90β, vascular endothelial growth factor, ATPase, Ca++ transporting, ubiquitous) in the nitric oxide pathway (NOP) were targets for THF-diols. Chromatin immunoprecipitation studies suggest THF-diols modify of histone H4 acetylation at the caveolin 1 promoter via an epigenetic mechanism. These findings are consistent with the well-known involvement of NOP genes in cell proliferation and sexual behavior.

KW - Endocrine disruptor

KW - Growth regulation

KW - Human breast cancer

KW - Microarray

KW - Nitric oxide

KW - Reproduction

KW - Tetrahydrofurandiol

UR - https://www.scopus.com/pages/publications/42749083708

U2 - 10.1016/j.canlet.2008.01.048

DO - 10.1016/j.canlet.2008.01.048

M3 - Article

C2 - 18346845

AN - SCOPUS:42749083708

SN - 0304-3835

VL - 264

SP - 265

EP - 273

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Regulation of the nitric oxide pathway genes by tetrahydrofurandiols: Microarray analysis of MCF-7 human breast cancer cells

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Keywords

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