Regulation of N-region diversity in antigen receptors through thymocyte differentiation and thymus ontogeny

M. Bogue, S. Gilfillan, C. Benoist, D. Mathis

Research output: Contribution to journalArticle

129 Scopus citations

Abstract

The random addition of 'N nucleotides' by terminal deoxynucleotidyltransferase (TdT) is an important component of the diversity of T-cell receptor genes. We have investigated the expression of TdT during thymocyte differentiation and thymus ontogeny. TdT gene transcripts are confined to immature thymocytes of the cortex, being downregulated concomitantly with recombination-activating gene transcripts after positive selection of mature medullary T cells. According to in situ hybridization, TdT RNA is absent from the neonatal thymus, but it appears 3 to 5 days after birth, just before the appearance of significant N-region diversity in T-cell receptor junctional sequences but clearly after the thymus attains competence at clonal deletion.

Original languageEnglish
Pages (from-to)11011-11015
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number22
DOIs
StatePublished - Nov 26 1992
Externally publishedYes

Keywords

  • T-cell receptor
  • T-cell repertoire
  • T-lymphocyte maturation
  • terminal deoxynucleotidyltransferase

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