Regulation of monocyte cell fate by blood vessels mediated by Notch signalling

Jaba Gamrekelashvili; Roberto Giagnorio; Jasmin Jussofie; Oliver Soehnlein; Johan Duchene; Carlos G. Briseño; Saravana K. Ramasamy; Kashyap Krishnasamy; Anne Limbourg; Tamar Kapanadze; Chieko Ishifune; Rabea Hinkel; Freddy Radtke; Lothar J. Strobl; Ursula Zimber-Strobl; L. Christian Napp; Johann Bauersachs; Hermann Haller; Koji Yasutomo; Christian Kupatt; Kenneth M. Murphy; Ralf H. Adams; Christian Weber; Florian P. Limbourg

doi:10.1038/ncomms12597

Regulation of monocyte cell fate by blood vessels mediated by Notch signalling

Jaba Gamrekelashvili, Roberto Giagnorio, Jasmin Jussofie, Oliver Soehnlein, Johan Duchene, Carlos G. Briseño, Saravana K. Ramasamy, Kashyap Krishnasamy, Anne Limbourg, Tamar Kapanadze, Chieko Ishifune, Rabea Hinkel, Freddy Radtke, Lothar J. Strobl, Ursula Zimber-Strobl, L. Christian Napp, Johann Bauersachs, Hermann Haller, Koji Yasutomo, Christian KupattKenneth M. Murphy, Ralf H. Adams, Christian Weber, Florian P. Limbourg

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Abstract

A population of monocytes, known as Ly6C^lo monocytes, patrol blood vessels by crawling along the vascular endothelium. Here we show that endothelial cells control their origin through Notch signalling. Using combinations of conditional genetic deletion strategies and cell-fate tracking experiments we show that Notch2 regulates conversion of Ly6C^hi monocytes into Ly6C^lo monocytes in vivo and in vitro, thereby regulating monocyte cell fate under steady-state conditions. This process is controlled by Notch ligand delta-like 1 (Dll1) expressed by a population of endothelial cells that constitute distinct vascular niches in the bone marrow and spleen in vivo, while culture on recombinant DLL1 induces monocyte conversion in vitro. Thus, blood vessels regulate monocyte conversion, a form of committed myeloid cell fate regulation.

Original language	English
Article number	12597
Journal	Nature communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms12597
State	Published - Aug 31 2016

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Gamrekelashvili, J., Giagnorio, R., Jussofie, J., Soehnlein, O., Duchene, J., Briseño, C. G., Ramasamy, S. K., Krishnasamy, K., Limbourg, A., Kapanadze, T., Ishifune, C., Hinkel, R., Radtke, F., Strobl, L. J., Zimber-Strobl, U., Napp, L. C., Bauersachs, J., Haller, H., Yasutomo, K., ... Limbourg, F. P. (2016). Regulation of monocyte cell fate by blood vessels mediated by Notch signalling. Nature communications, 7, [12597]. https://doi.org/10.1038/ncomms12597

@article{63734cf7c008462cb7f1e3b9472a9d8e,

title = "Regulation of monocyte cell fate by blood vessels mediated by Notch signalling",

abstract = "A population of monocytes, known as Ly6Clo monocytes, patrol blood vessels by crawling along the vascular endothelium. Here we show that endothelial cells control their origin through Notch signalling. Using combinations of conditional genetic deletion strategies and cell-fate tracking experiments we show that Notch2 regulates conversion of Ly6Chi monocytes into Ly6Clo monocytes in vivo and in vitro, thereby regulating monocyte cell fate under steady-state conditions. This process is controlled by Notch ligand delta-like 1 (Dll1) expressed by a population of endothelial cells that constitute distinct vascular niches in the bone marrow and spleen in vivo, while culture on recombinant DLL1 induces monocyte conversion in vitro. Thus, blood vessels regulate monocyte conversion, a form of committed myeloid cell fate regulation.",

author = "Jaba Gamrekelashvili and Roberto Giagnorio and Jasmin Jussofie and Oliver Soehnlein and Johan Duchene and Brise{\~n}o, {Carlos G.} and Ramasamy, {Saravana K.} and Kashyap Krishnasamy and Anne Limbourg and Tamar Kapanadze and Chieko Ishifune and Rabea Hinkel and Freddy Radtke and Strobl, {Lothar J.} and Ursula Zimber-Strobl and Napp, {L. Christian} and Johann Bauersachs and Hermann Haller and Koji Yasutomo and Christian Kupatt and Murphy, {Kenneth M.} and Adams, {Ralf H.} and Christian Weber and Limbourg, {Florian P.}",

note = "Funding Information: We thank Sylvana Henschen and Stefan Sablotny for excellent technical assistance; Achim Gossler for Dll1+lacZ mice; the Central Animal Facility, Research Core Facility Cell Sorting, Research Core unit Laser Microscopy of Hannover Medical School for excellent support. This research has been funded by grants from DFG (Li948-5/1), BMBF (01GU1105B), IFB-Tx (01EO1302) and GIF (1089) to F.P.L. and SFB1054-B04 to C.W. Publisher Copyright: {\textcopyright} 2016 The Author(s).",

year = "2016",

month = aug,

day = "31",

doi = "10.1038/ncomms12597",

language = "English",

volume = "7",

journal = "Nature Communications",

issn = "2041-1723",

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Gamrekelashvili, J, Giagnorio, R, Jussofie, J, Soehnlein, O, Duchene, J, Briseño, CG, Ramasamy, SK, Krishnasamy, K, Limbourg, A, Kapanadze, T, Ishifune, C, Hinkel, R, Radtke, F, Strobl, LJ, Zimber-Strobl, U, Napp, LC, Bauersachs, J, Haller, H, Yasutomo, K, Kupatt, C, Murphy, KM, Adams, RH, Weber, C & Limbourg, FP 2016, 'Regulation of monocyte cell fate by blood vessels mediated by Notch signalling', Nature communications, vol. 7, 12597. https://doi.org/10.1038/ncomms12597

TY - JOUR

T1 - Regulation of monocyte cell fate by blood vessels mediated by Notch signalling

AU - Gamrekelashvili, Jaba

AU - Giagnorio, Roberto

AU - Jussofie, Jasmin

AU - Soehnlein, Oliver

AU - Duchene, Johan

AU - Briseño, Carlos G.

AU - Ramasamy, Saravana K.

AU - Krishnasamy, Kashyap

AU - Limbourg, Anne

AU - Kapanadze, Tamar

AU - Ishifune, Chieko

AU - Hinkel, Rabea

AU - Radtke, Freddy

AU - Strobl, Lothar J.

AU - Zimber-Strobl, Ursula

AU - Napp, L. Christian

AU - Bauersachs, Johann

AU - Haller, Hermann

AU - Yasutomo, Koji

AU - Kupatt, Christian

AU - Murphy, Kenneth M.

AU - Adams, Ralf H.

AU - Weber, Christian

AU - Limbourg, Florian P.

N1 - Funding Information: We thank Sylvana Henschen and Stefan Sablotny for excellent technical assistance; Achim Gossler for Dll1+lacZ mice; the Central Animal Facility, Research Core Facility Cell Sorting, Research Core unit Laser Microscopy of Hannover Medical School for excellent support. This research has been funded by grants from DFG (Li948-5/1), BMBF (01GU1105B), IFB-Tx (01EO1302) and GIF (1089) to F.P.L. and SFB1054-B04 to C.W. Publisher Copyright: © 2016 The Author(s).

PY - 2016/8/31

Y1 - 2016/8/31

N2 - A population of monocytes, known as Ly6Clo monocytes, patrol blood vessels by crawling along the vascular endothelium. Here we show that endothelial cells control their origin through Notch signalling. Using combinations of conditional genetic deletion strategies and cell-fate tracking experiments we show that Notch2 regulates conversion of Ly6Chi monocytes into Ly6Clo monocytes in vivo and in vitro, thereby regulating monocyte cell fate under steady-state conditions. This process is controlled by Notch ligand delta-like 1 (Dll1) expressed by a population of endothelial cells that constitute distinct vascular niches in the bone marrow and spleen in vivo, while culture on recombinant DLL1 induces monocyte conversion in vitro. Thus, blood vessels regulate monocyte conversion, a form of committed myeloid cell fate regulation.

AB - A population of monocytes, known as Ly6Clo monocytes, patrol blood vessels by crawling along the vascular endothelium. Here we show that endothelial cells control their origin through Notch signalling. Using combinations of conditional genetic deletion strategies and cell-fate tracking experiments we show that Notch2 regulates conversion of Ly6Chi monocytes into Ly6Clo monocytes in vivo and in vitro, thereby regulating monocyte cell fate under steady-state conditions. This process is controlled by Notch ligand delta-like 1 (Dll1) expressed by a population of endothelial cells that constitute distinct vascular niches in the bone marrow and spleen in vivo, while culture on recombinant DLL1 induces monocyte conversion in vitro. Thus, blood vessels regulate monocyte conversion, a form of committed myeloid cell fate regulation.

UR - http://www.scopus.com/inward/record.url?scp=84984985562&partnerID=8YFLogxK

U2 - 10.1038/ncomms12597

DO - 10.1038/ncomms12597

M3 - Article

C2 - 27576369

AN - SCOPUS:84984985562

SN - 2041-1723

VL - 7

JO - Nature Communications

JF - Nature Communications

M1 - 12597

ER -

Regulation of monocyte cell fate by blood vessels mediated by Notch signalling

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