TY - JOUR
T1 - Regulation of expression of the c-sis proto-oncogene
AU - Ratner, Lee
N1 - Funding Information:
ACKNOWLEDGEMENTS I thank Jim Thielan, Nancy Vander Heyden, Peter Westervelt, Josephs DiGiuseppe, and Cathy Polera for technical assistance, and Jeffrey Milbrandt, Irvin Chen, David Derse, Craig Rosen, Stephen Josephs, and Suresh Arya for plasmid clones. I thank Tim Ley for critical review of the manuscript. This work was supported by a Washington University-Monsanto collaborative research fund. Lee Ratner is a Hartford Foundation fellow.
PY - 1989/6/12
Y1 - 1989/6/12
N2 - Regulation of expression of platelet derived growth factor polypeptide B encoded by the c-sis proto-oncogene is important in a number of physiological and pathological conditions. Sequences in the 1028 nucleotide long 5' untranslated region of the c-sis mRNA were found to inhibit protein synthesis. The inhibition is relieved by deletion of nucleotides 154-378 or 398-475. Sequences within 375 nucleotides upstream of the RNA initiation site are important for transcriptional activity. Sequences in two portions of this region, between -375 and -235 nucletoides and between -235 and -99 nucleotides relative to the RNA CAP site are important for full activity. A transcriptional enhancer activity is demonstrated by its ability to increase the activity of the human T lymphotropic virus type (HTLV) I promoter at a distance and in an orientation-independent manner. Furthermore, sequences upstream of the c-sis RNA CAP site respond to the HTLV I transactivator protein to increase RNA synthesis from either the c-sis or HTLV I promoter.
AB - Regulation of expression of platelet derived growth factor polypeptide B encoded by the c-sis proto-oncogene is important in a number of physiological and pathological conditions. Sequences in the 1028 nucleotide long 5' untranslated region of the c-sis mRNA were found to inhibit protein synthesis. The inhibition is relieved by deletion of nucleotides 154-378 or 398-475. Sequences within 375 nucleotides upstream of the RNA initiation site are important for transcriptional activity. Sequences in two portions of this region, between -375 and -235 nucletoides and between -235 and -99 nucleotides relative to the RNA CAP site are important for full activity. A transcriptional enhancer activity is demonstrated by its ability to increase the activity of the human T lymphotropic virus type (HTLV) I promoter at a distance and in an orientation-independent manner. Furthermore, sequences upstream of the c-sis RNA CAP site respond to the HTLV I transactivator protein to increase RNA synthesis from either the c-sis or HTLV I promoter.
UR - https://www.scopus.com/pages/publications/0024322412
U2 - 10.1093/nar/17.11.4101
DO - 10.1093/nar/17.11.4101
M3 - Article
C2 - 2740212
AN - SCOPUS:0024322412
SN - 0305-1048
VL - 17
SP - 4101
EP - 4115
JO - Nucleic acids research
JF - Nucleic acids research
IS - 11
ER -