TY - JOUR
T1 - Regulation of expression of B7 by murine langerhans cells
T2 - A direct relationship between B7 mRNA levels and the level of surface expression of B7 by Langerhans cells
AU - Lee, Min Geol
AU - Borkowski, Teresa A.
AU - Udey, Mark C.
PY - 1993/12
Y1 - 1993/12
N2 - Cultured BALB/c epidermal Langerhans cells express high levels of the costimulatory molecule B7 on their surfaces relative to levels expressed on fresh Langerhans cells. Quantitation of relative amounts of B7 mRNA in fresh epidermal cells and cultured epidermal cells following amplification of mRNA signals via reverse transcriptase-polymerase chain reaction, hybridization of PCR products with radiolabeled internal oligonucleotide probes, resolution of hybrids in non-denaturing polyacrylamide gels, and detection by autoradiography revealed dramatically (approximately one thousandfold) higher levels of B7 mRNA in cultured epidermal cells (10-40% I-A+) as compared with fresh epidermal cells (1-4% I-A+). Levels of B7 mRNA in cultured epidermal cells were also substantially greater than those detected in a reference B lymphoma cell line (CH-1). Analysis of B7 mRNA expression in subpopulations of cultured epidermal cells demonstrated that essentially all of the B7 mRNA was present in Langerhans cells; cells bearing I-A and CD45 antigens. Cultured keratinocytes did not contain appreciable amounts of B7 mRNA. These results are consistent with previous data regarding surface expression of B7 by cLC and also demonstrate that fLC are essentially devoid of B7 mRNA and surface protein.
AB - Cultured BALB/c epidermal Langerhans cells express high levels of the costimulatory molecule B7 on their surfaces relative to levels expressed on fresh Langerhans cells. Quantitation of relative amounts of B7 mRNA in fresh epidermal cells and cultured epidermal cells following amplification of mRNA signals via reverse transcriptase-polymerase chain reaction, hybridization of PCR products with radiolabeled internal oligonucleotide probes, resolution of hybrids in non-denaturing polyacrylamide gels, and detection by autoradiography revealed dramatically (approximately one thousandfold) higher levels of B7 mRNA in cultured epidermal cells (10-40% I-A+) as compared with fresh epidermal cells (1-4% I-A+). Levels of B7 mRNA in cultured epidermal cells were also substantially greater than those detected in a reference B lymphoma cell line (CH-1). Analysis of B7 mRNA expression in subpopulations of cultured epidermal cells demonstrated that essentially all of the B7 mRNA was present in Langerhans cells; cells bearing I-A and CD45 antigens. Cultured keratinocytes did not contain appreciable amounts of B7 mRNA. These results are consistent with previous data regarding surface expression of B7 by cLC and also demonstrate that fLC are essentially devoid of B7 mRNA and surface protein.
KW - CD28
KW - CTLA-4
KW - accessory cells
KW - costimulatory molecules
UR - http://www.scopus.com/inward/record.url?scp=0027381191&partnerID=8YFLogxK
U2 - 10.1111/1523-1747.ep12371712
DO - 10.1111/1523-1747.ep12371712
M3 - Article
C2 - 7504029
AN - SCOPUS:0027381191
SN - 0022-202X
VL - 101
SP - 883
EP - 886
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 6
ER -