Regulation of embryonic endochondral ossification by Smurf2

Qiuqian Wu, Meina Wang, Michael J. Zuscik, Di Chen, Regis J. O'Keefe, Randy N. Rosier

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Smurff2 is an E3 ubiquitin ligase that targets TGF-β receptor activated Smad2 and Smad3 for the proteasome in primary articular chondrocytes, thus stimulating their hypertrophic differentiation. Comparatively, how Smurf2 functions in growth plate chondrocytes in a developing long bone is an open question. In this study, we measured the mRNA levels of endogenous Smurf2 and type X collagen in chick growth plate at different embryonic stages to monitor the correlation between the level of Smurf2 expression and chondrocyte maturational stage. We found that high levels of Smurf2 were associated with the differentiative and proliferative stages, while Smurff2 levels were thereafter decreased as the chondrocytes matured toward hypertrophy. In addition, we injected Smurf2-RCAS into chick wing buds at HH stage 20-23 and examined how the ectopic overexpression of Smurf2 in condensing chondrogenic mesenchyme affects the subsequent process of chondrocyte maturation and ossification during embryonic development. Histological analysis showed that overexpression of Smurff2 in a developing wing bud accelerated chondrocyte maturation and endochondral ossification, which may result from a decrease in TGF-β signaling in the infected chondrocytes with Smurf2-RCAS.

Original languageEnglish
Pages (from-to)704-712
Number of pages9
JournalJournal of Orthopaedic Research
Volume26
Issue number5
DOIs
StatePublished - May 1 2008
Externally publishedYes

Keywords

  • Chondrocyte
  • Embryonic development
  • Endochondral ossification
  • Smurf2
  • TGF-β signaling

Fingerprint Dive into the research topics of 'Regulation of embryonic endochondral ossification by Smurf2'. Together they form a unique fingerprint.

  • Cite this