Regulation of CapZ, an Actin Capping Protein of Chicken Muscle, by Anionic Phospholipids

Steven G. Heiss, John A. Cooper

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

Chicken muscle CapZ, a member of the capping protein family of actin-binding proteins, binds to the barbed end of actin filaments and nucleates actin polymerization. No regulation of the capping protein family has been described. We report that micelles of phosphatidylinositol 4,5-bisphosphate (PIP2) bind to CapZ and completely inhibit its ability to affect actin polymerization as measured by several independent assays. Higher concentrations of other anionic phospholipids also completely inhibit the activity of CapZ. Neutral phospholipids have no effect. Mixed vesicles of PIP2 with phosphatidylcholine or phosphatidyl-ethanolamine also inhibit CapZ, but addition of Triton X-100 both prevents and reverses PIP2's inhibition of CapZ.

Original languageEnglish
Pages (from-to)8753-8758
Number of pages6
JournalBiochemistry
Volume30
Issue number36
DOIs
StatePublished - Sep 1 1991

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