Regulation of antigen-presentation-I. IFN-γ induces antigen-presenting properties on B cells

C. M. Hawrylowicz, E. R. Unanue

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

B cells require activation to efficiently present Ag to T cells. In agreement with this earlier observation we show that live, mitomycin C-treated B cells, but not B cells fixed in paraformaldehyde, stimulated the growth of allogeneic T cells in the primary MLR. However, if B cells were cultured with anti-Ig antibodies and IFN-γ before fixation they acquired excellent T cell stimulatory activity. Neither reagent alone conferred this novel co-stimulatory function on the B cell surface. The activity induced by both stimuli was not attributed to an increase expression of class II-MHC molecules or Il-1. IL-2 or IL-4, in combination with anti-Ig, also induced B cell stimulatory activity, but were less effective than IFN-γ. TNF failed to stimulate B cells, but synergized with IFN-γ in the induction of this activity. These studies therefore demonstrate an important role for lymphokines in modulating B cell Ag-presenting activity as well as the acquisition by B cells of a novel co-stimulatory surface activity.

Original languageEnglish
Pages (from-to)4083-4088
Number of pages6
JournalJournal of Immunology
Volume141
Issue number12
StatePublished - Dec 1 1988

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