Regulation of 25 hydroxyvitamin D3 1-Hydroxylase in serum-free monolayer culture of mouse kidney

M. Fukase, S. J. Birge, L. Rifas, L. V. Avioli, L. R. Chase

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Primary monolayer cultures of cells from normal mouse kidney were initiated and maintained in serum-free medium supplemented with insulin, transferrin and prostaglandin E1. Renal epithelial cells grow to confluence without detectable growth of fibroblasts. These cells contain an active 25(OH)D 3 1-hydroxylase with half-maximal formation of 1,25(OH>2D3 achieved at a substrate concentration of 13.3 nM. Activity of this enzyme is increased by low calcium medium or parathyroid hormone and decreased by high calcium medium, high phosphate medium or 1,25(HO)2D3.

Original languageEnglish
Pages (from-to)1073-1075
Number of pages3
JournalEndocrinology
Volume110
Issue number3
DOIs
StatePublished - Mar 1982

Fingerprint

Dive into the research topics of 'Regulation of 25 hydroxyvitamin D3 1-Hydroxylase in serum-free monolayer culture of mouse kidney'. Together they form a unique fingerprint.

Cite this