Regulation of 25 hydroxyvitamin D3 1-Hydroxylase in serum-free monolayer culture of mouse kidney

M. Fukase; S. J. Birge; L. Rifas; L. V. Avioli; L. R. Chase

doi:10.1210/endo-110-3-1073

Regulation of 25 hydroxyvitamin D₃ 1-Hydroxylase in serum-free monolayer culture of mouse kidney

M. Fukase, S. J. Birge, L. Rifas, L. V. Avioli, L. R. Chase

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Abstract

Primary monolayer cultures of cells from normal mouse kidney were initiated and maintained in serum-free medium supplemented with insulin, transferrin and prostaglandin E₁. Renal epithelial cells grow to confluence without detectable growth of fibroblasts. These cells contain an active 25(OH)D 3 1-hydroxylase with half-maximal formation of 1,25(OH>₂D₃ achieved at a substrate concentration of 13.3 nM. Activity of this enzyme is increased by low calcium medium or parathyroid hormone and decreased by high calcium medium, high phosphate medium or 1,25(HO)₂D₃.

Original language	English
Pages (from-to)	1073-1075
Number of pages	3
Journal	Endocrinology
Volume	110
Issue number	3
DOIs	https://doi.org/10.1210/endo-110-3-1073
State	Published - Mar 1982

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abstract = "Primary monolayer cultures of cells from normal mouse kidney were initiated and maintained in serum-free medium supplemented with insulin, transferrin and prostaglandin E1. Renal epithelial cells grow to confluence without detectable growth of fibroblasts. These cells contain an active 25(OH)D 3 1-hydroxylase with half-maximal formation of 1,25(OH>2D3 achieved at a substrate concentration of 13.3 nM. Activity of this enzyme is increased by low calcium medium or parathyroid hormone and decreased by high calcium medium, high phosphate medium or 1,25(HO)2D3.",

author = "M. Fukase and Birge, {S. J.} and L. Rifas and Avioli, {L. V.} and Chase, {L. R.}",

year = "1982",

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AU - Fukase, M.

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AU - Avioli, L. V.

AU - Chase, L. R.

PY - 1982/3

Y1 - 1982/3

N2 - Primary monolayer cultures of cells from normal mouse kidney were initiated and maintained in serum-free medium supplemented with insulin, transferrin and prostaglandin E1. Renal epithelial cells grow to confluence without detectable growth of fibroblasts. These cells contain an active 25(OH)D 3 1-hydroxylase with half-maximal formation of 1,25(OH>2D3 achieved at a substrate concentration of 13.3 nM. Activity of this enzyme is increased by low calcium medium or parathyroid hormone and decreased by high calcium medium, high phosphate medium or 1,25(HO)2D3.

AB - Primary monolayer cultures of cells from normal mouse kidney were initiated and maintained in serum-free medium supplemented with insulin, transferrin and prostaglandin E1. Renal epithelial cells grow to confluence without detectable growth of fibroblasts. These cells contain an active 25(OH)D 3 1-hydroxylase with half-maximal formation of 1,25(OH>2D3 achieved at a substrate concentration of 13.3 nM. Activity of this enzyme is increased by low calcium medium or parathyroid hormone and decreased by high calcium medium, high phosphate medium or 1,25(HO)2D3.

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Regulation of 25 hydroxyvitamin D₃ 1-Hydroxylase in serum-free monolayer culture of mouse kidney

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